Capillary electrochromatography with a neutral monolithic column for classification of analytes and determination of basic drugs in human serum
文献类型：期刊论文
| 作者 | Dong, J; Xie, CH; Tian, RJ; Wu, RN; Hu, JW; Zou, HF |
| 刊名 | electrophoresis
 |
| 出版日期 | 2005-09-01 |
| 卷号 | 26期号:18页码:3452-3459 |
| 关键词 | basic drugs electrochromatography electrophoretic mobility human serum neutral monolith |
| 英文摘要 | capillary electrochromatography (cec) using a neutral hydrophobic polymer-based monolithic column has been developed for classification of analytes based on their acidity and charges of group. the monolithic columns were prepared by in situ copolymerization of lauryl methacrylate and ethylene dimethacrylate without any charged monomers in the reaction mixture. the ionic analytes will only be driven by their electrophoretic mobilities and were separated on the basis of their differences in electrophoretic mobility and in hydrophobic interaction with the stationary phase. only negatively or positively charged analyte (acid or basic) migrated toward detection window in a single run by applying negative or positive voltage. the cec system was also applied for the analysis of basic drugs in human serum by internal standard method using acidic running buffer. the sample of human serum spiked with basic drugs was directly injected after a simple sample pretreatment. the calibration curves with regression coefficients (0.9995-0.9997) in the range of 0.318-80 mu g/ml were obtained with the limits of detection being below 0.15 mu g/ml. the intra- and inter-day precisions, determined as relative standard deviations, were less than 4.21%. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | porous polymer monoliths ; liquid-chromatography ; separation media ; stationary phases ; electrophoresis ; performance ; pressure ; driven |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000232351800006 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/139874]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dong, J,Xie, CH,Tian, RJ,et al. Capillary electrochromatography with a neutral monolithic column for classification of analytes and determination of basic drugs in human serum[J]. electrophoresis,2005,26(18):3452-3459. |
| APA | Dong, J,Xie, CH,Tian, RJ,Wu, RN,Hu, JW,&Zou, HF.(2005).Capillary electrochromatography with a neutral monolithic column for classification of analytes and determination of basic drugs in human serum.electrophoresis,26(18),3452-3459. |
| MLA | Dong, J,et al."Capillary electrochromatography with a neutral monolithic column for classification of analytes and determination of basic drugs in human serum".electrophoresis 26.18(2005):3452-3459. |
入库方式： OAI收割
来源：大连化学物理研究所
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