Optimization of Saccharomyces cerevisiae culture in alginate-chitosan-alginate microcapsule
文献类型：期刊论文
| 作者 | Qi, WT; Yu, WT; Xie, YB; Ma, XJ |
| 刊名 | biochemical engineering journal
 |
| 出版日期 | 2005-09-01 |
| 卷号 | 25期号:2页码:151-157 |
| 关键词 | microencapsulation ACA microcapsule Saccharomyces cerevisiae cell growth |
| 英文摘要 | microencapsulation is one of the promising methods for microorganism immobilization. the parameters of microencapsulation have impact on the growth and performance of microencapsulated microorganism. in this paper, the effects of microcapsules core state (solid or liquid), initial cell density (1.5 x 10(7), 3 x 10(6) and 3 x 10(5) cells/ml of microcapsules), microcapsule diameter (200, 500, 600 and 700 mu m) and membrane formation times (0, 5, 15 and 30 min) on cell growth, including proliferating capacity, metabolic activity and product secretion of saccharomyces cerevisiae, cultured in alginate-chitosan-alginate (aca) microcapsule, were investigated. the results showed that there was no significant difference in cell growth of microencapsulated cells, whether the core of the microcapsules was solid or liquefied. increase in inoculate cell density shortened the lag phase time of cell growth, while cell density obtained 25 times of the initial density of 3 x 10(6) cells/ml of microcapsule, which was the highest. increase in microcapsules size had no significant impact on cell proliferation, metabolism and product secretion, but the leakage of the bacteria was the least when the microcapsule size was 600 mu m in diameter. the increase in membrane formation time reduced the leakage of cells. it was demonstrated that the optimized parameters for microencapsulated s. cerevisiae culture were initial cell density of 3 x 10(6) cells/ml of microcapsule, microcapsule size of 600 mu m in diameter, and membrane formation time of 15 min. this study provides useful information for intestinal delivery of therapeutic agents from genetically modified food-grade microorganisms, using microencapsulation. (c) 2005 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; technology |
| 类目[WOS] | biotechnology & applied microbiology ; engineering, chemical |
| 研究领域[WOS] | biotechnology & applied microbiology ; engineering |
| 关键词[WOS] | artificial cells ; microencapsulation ; encapsulation ; membrane ; survival ; delivery ; beads |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000231493900006 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/139878]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Sci & Technol, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Grad Sch, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, WT,Yu, WT,Xie, YB,et al. Optimization of Saccharomyces cerevisiae culture in alginate-chitosan-alginate microcapsule[J]. biochemical engineering journal,2005,25(2):151-157. |
| APA | Qi, WT,Yu, WT,Xie, YB,&Ma, XJ.(2005).Optimization of Saccharomyces cerevisiae culture in alginate-chitosan-alginate microcapsule.biochemical engineering journal,25(2),151-157. |
| MLA | Qi, WT,et al."Optimization of Saccharomyces cerevisiae culture in alginate-chitosan-alginate microcapsule".biochemical engineering journal 25.2(2005):151-157. |
入库方式： OAI收割
来源：大连化学物理研究所
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