On-line isotachophoretic preconcentration and gel electrophoretic separation of sodium dodecyl sulfate-proteins on a microchip
文献类型：期刊论文
| 作者 | Huang, HQ; Xu, F; Dai, ZP; Lin, BC |
| 刊名 | electrophoresis
 |
| 出版日期 | 2005-06-01 |
| 卷号 | 26期号:11页码:2254-2260 |
| 关键词 | gel electrophoresis isotachophoresis miniaturization on-line preconcentration sodium dodecyl sulfate-proteins |
| 英文摘要 | a microchip for integrated isotachophoretic (itp) preconcentration with gel electrophoretic (ge) separation to decrease the detectable concentration of sodium dodecyl sulfate (sds)-proteins was developed. each channel of the chip was designed with a long sample injection channel to increase the sample loading and allow stacking the sample into a narrow zone using discontinuous itp buffers. the preconcentrated sample was separated in ge mode in sieving polymer solutions. all the analysis steps including injection, preconcentration, and separation of the itp-ge process were performed continuously, controlled by a high-voltage power source with sequential voltage switching between the analysis steps. without deteriorating the peak resolution, four sds-protein analyses with integrated itp-ge system resulted in a decreased detectable concentration of similar to 40-fold compared to the ge mode only. a good calibration curve for molecular weights of sids-proteins indicated that the integrated itp-ge system can be used for qualitative analysis of unknown protein samples. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | total analysis systems ; column-coupling chip ; capillary zone electrophoresis ; microfluidic devices ; conductivity detection ; planar chip ; channels ; complexes ; samples ; dna |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000229901500023 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/139915]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Grad Sch, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, HQ,Xu, F,Dai, ZP,et al. On-line isotachophoretic preconcentration and gel electrophoretic separation of sodium dodecyl sulfate-proteins on a microchip[J]. electrophoresis,2005,26(11):2254-2260. |
| APA | Huang, HQ,Xu, F,Dai, ZP,&Lin, BC.(2005).On-line isotachophoretic preconcentration and gel electrophoretic separation of sodium dodecyl sulfate-proteins on a microchip.electrophoresis,26(11),2254-2260. |
| MLA | Huang, HQ,et al."On-line isotachophoretic preconcentration and gel electrophoretic separation of sodium dodecyl sulfate-proteins on a microchip".electrophoresis 26.11(2005):2254-2260. |
入库方式： OAI收割
来源：大连化学物理研究所
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