Characterization of small-molecule-biomicromolecule interactions: From simple to complex
文献类型：期刊论文
| 作者 | Tian, RJ; Xu, SY; Lei, XY; Jin, WH; Ye, ML; Zou, HF |
| 刊名 | trac-trends in analytical chemistry
 |
| 出版日期 | 2005-10-01 |
| 卷号 | 24期号:9页码:810-825 |
| 关键词 | analytical methodologies library screening multiple recognition systems simple ligand-receptor binding small-molecule-biomacromolecule interaction |
| 英文摘要 | the interactions between small molecules and biomacromolecules constitute one of the most important parts of interconnected biological networks. to understand the recognition mechanisms of these interactions is of vital importance for characterizing the expression and the function of related biomacromolecules and further discovering drug candidates for the pharmaceutical industry and biomarkers for clinical diagnosis. here, we summarize the general methodology used in these studies, mainly including chromatographic, electromigration and mass spectrometric methods. we present the latest applications of these methods for elucidating simple binding of plasma protein, enzyme, receptor and dna/rna with small molecules. we also present recognition studies based on multi-component systems (e.g., phage display, oligonucleotides, chemiproteomics and traditional chinese medicines), which should be more challenging in both analytical tools and methodology and the function of biological systems. (c) 2005 elsevier ltd. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | human serum-albumin ; ionization mass-spectrometry ; performance liquid-chromatography ; affinity capillary-electrophoresis ; cdna-phage display ; immobilized-enzyme reactor ; surface-plasmon resonance ; dissociation-constants ; competitive-binding ; stationary phases |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000232970400010 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/139931]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tian, RJ,Xu, SY,Lei, XY,et al. Characterization of small-molecule-biomicromolecule interactions: From simple to complex[J]. trac-trends in analytical chemistry,2005,24(9):810-825. |
| APA | Tian, RJ,Xu, SY,Lei, XY,Jin, WH,Ye, ML,&Zou, HF.(2005).Characterization of small-molecule-biomicromolecule interactions: From simple to complex.trac-trends in analytical chemistry,24(9),810-825. |
| MLA | Tian, RJ,et al."Characterization of small-molecule-biomicromolecule interactions: From simple to complex".trac-trends in analytical chemistry 24.9(2005):810-825. |
入库方式： OAI收割
来源：大连化学物理研究所
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