中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Induced meiotic gynogenesis in tench, Tinca tinca (L.) using irradiated heterogenic sperm

文献类型:期刊论文

作者Wang, JX; Liu, HJ; Min, WQ; Tong, JG; Guan, M; Han, YZ; Gong, LJ; Huang, Z; Ren, J; Zhang, JP
刊名AQUACULTURE INTERNATIONAL
出版日期2006-02-01
卷号14期号:1-2页码:35-42
关键词heterogenic sperm meiotic gynogenesis Tinca tinca
ISSN号0967-6120
英文摘要Effects of the starting time and duration of cold shock, as well as the source of heterogenic sperm on the percentage of viable gynogenic larvae (PVGL) in tench were studied. The DNA in sperm of red common carp (Cyprinus carpio var singuonensis) was inactivated by ultraviolet radiation prior to use to induce meiotic gynogenetic development in tench. In experiment 1, tench eggs were cold-shocked for 30 min starting at 1, 3, 5, 7, 10 and 15 min post activation. In experiment 2, cold shock began 5 min after activation and lasted for 10, 20, 30, 40, 50 min, respectively. Each experiment was run in triplicate using 3 tench females, and one group not treated with cold shock was included in each experiment to serve as a control group. In experiment 3, sperm of bigmouth buffalo (Ictiobus cyprinellus), red common carp or grass carp (Ctenopharyngodon idella) was used to induce gynogenesis in tench with a 20-min cold shock starting 5 min post activation. The results showed that PVGL from the control group was very low (0.11-0.47%). In experiment 1, the highest average PVGL (9.60%) was observed when cold shock treatment was applied 5 min post activation. When cold shock treatment was started 5 min post activation, duration of cold shock affected PVGL. Cold shock lasting 20 min resulted in the highest average PVGL (12. 57%) among the selected duration of cold shock studied in experiment 2. The average PVGL was 2.3, 8.6, and 9.3%, respectively, for eggs induced by sperm of bigmouth buffalo, red common carp and grass carp. Average PVGL was significantly lower for eggs induced by sperm of bigmouth buffalo, compared with that for eggs induced by sperm of the other two species. However, average PVGL were similar for eggs induced by sperm of red common carp and grass carp. In summary, the optimal conditions for gynogenesis in tench include the use of irradiated sperm of grass carp to activate the eggs and cold shock of 20 min starting 5 min post activation. Since female tench grow much faster to a larger size than male tench, gynogenesis of tench holds great potential for production enhancement.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Fisheries
研究领域[WOS]Fisheries
收录类别SCI ; ISTP
语种英语
WOS记录号WOS:000234692500005
公开日期2015-11-10
源URL[http://ir.ihb.ac.cn/handle/342005/27218]  
专题水生生物研究所_中科院水生所知识产出(2009年前)_期刊论文
作者单位1.Hubei Prov Fisheries Res Inst, Wuchang 430071, Hubei, Peoples R China
2.Acad Sinica, Inst Hydrobiol, Wuhan 430072, Peoples R China
3.Zhaoqing Goldeatech Co Ltd, Zhaoqing 526200, Peoples R China
推荐引用方式
GB/T 7714
Wang, JX,Liu, HJ,Min, WQ,et al. Induced meiotic gynogenesis in tench, Tinca tinca (L.) using irradiated heterogenic sperm[J]. AQUACULTURE INTERNATIONAL,2006,14(1-2):35-42.
APA Wang, JX.,Liu, HJ.,Min, WQ.,Tong, JG.,Guan, M.,...&Zheng, HP.(2006).Induced meiotic gynogenesis in tench, Tinca tinca (L.) using irradiated heterogenic sperm.AQUACULTURE INTERNATIONAL,14(1-2),35-42.
MLA Wang, JX,et al."Induced meiotic gynogenesis in tench, Tinca tinca (L.) using irradiated heterogenic sperm".AQUACULTURE INTERNATIONAL 14.1-2(2006):35-42.

入库方式: OAI收割

来源:水生生物研究所

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