On-line combination of capillay isoelectric focusing and capillary non-gel sieving electrophoresis using a hollow-fiber membrane interface: a novel two-dimensional separation system for proteins
文献类型：期刊论文
| 作者 | Liu, HC; Yang, C; Yang, Q; Zhang, WB; Zhang, YK |
| 刊名 | journal of chromatography b-analytical technologies in the biomedical and life sciences
 |
| 出版日期 | 2005-03-05 |
| 卷号 | 817期号:1页码:119-126 |
| 关键词 | capillary isoelectric focusing capillary non-gel sieving electrophoresis hollow-fiber membrane interface proteins |
| 英文摘要 | a novel two-dimensional (2d) separation system for proteins was reported. in the system, a piece of dialysis hollow-fiber membrane was employed as the interface for on-line combination of capillary isoelectric focusing (cief) and capillary non-gel sieving electrophoresis (cngse). the system is similar equivalent to two-dimensional polyacrylamide gel electrophoresis (2d page), by transferring the principal of 2d page separation to the capillary format. proteins were focused and separated in first dimension cief based on their differences in isoelectric points (pis). focused protein zones was transferred to the dialysis hollow-fiber interface, where proteins hydrophobically complexed with sodium dodecyl sulfate (sds). the negatively charged proteins were electromigrated and further resolved by their differences in size in the second dimension cngse, in which dextran solution, a replaceable sieving matrix instead of cross-linked polyacrylamide gel was employed for size-dependent separation of proteins. the combination of the two techniques was attributed to high efficiency of the dialysis membrane interface. the feasibility and the orthogonality of the combined cief-cngse separation technique, an important factor for maximizing peak capacity or resolution elements, were demonstrated by examining each technique independently for the separation of hemoglobin and protein mixtures excreting from lung cancer cells of rat. the 2d separation strategy was found to greatly increase the resolving power and overall peak capacity over those obtained for either dimension alone. (c) 2004 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | high-performance electrophoresis ; phase liquid-chromatography ; zone-electrophoresis ; mass-spectrometry ; polyacrylamide-gel ; peptides ; proteomics ; device |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000226919100013 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/139933]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Natl Chromatograph R&A Ctr, Dalian Inst Chem Phys, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, HC,Yang, C,Yang, Q,et al. On-line combination of capillay isoelectric focusing and capillary non-gel sieving electrophoresis using a hollow-fiber membrane interface: a novel two-dimensional separation system for proteins[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2005,817(1):119-126. |
| APA | Liu, HC,Yang, C,Yang, Q,Zhang, WB,&Zhang, YK.(2005).On-line combination of capillay isoelectric focusing and capillary non-gel sieving electrophoresis using a hollow-fiber membrane interface: a novel two-dimensional separation system for proteins.journal of chromatography b-analytical technologies in the biomedical and life sciences,817(1),119-126. |
| MLA | Liu, HC,et al."On-line combination of capillay isoelectric focusing and capillary non-gel sieving electrophoresis using a hollow-fiber membrane interface: a novel two-dimensional separation system for proteins".journal of chromatography b-analytical technologies in the biomedical and life sciences 817.1(2005):119-126. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。