Purification and characterization of a novel stable ginsenoside Rb-1-hydrolyzing beta-D-glucosidase from China white jade snail
文献类型：期刊论文
| 作者 | Luan, Hongwei; Liu, Xin; Qi, Xiaohui; Hu, Ying; Hao, Dacheng; Cui, Yu; Yang, Ling |
| 刊名 | process biochemistry
 |
| 出版日期 | 2006-09-01 |
| 卷号 | 41期号:9页码:1974-1980 |
| 关键词 | beta-D-glucosidase thermo- and pH-stability ginsenoside Rb-1 ginsenoside Rd purification China white jade snail |
| 英文摘要 | a highly stable beta-glucosidase from the viscera of china white jade snail was purified to apparent homogeneity. this purified glucosidase consisted of two identical subunits with a native molecular mass of approximately 230 kda. the maximal activity to p-nitrophenyl-beta-(d)-glucopyranoside (pnpg) occurred at 70 degrees c and ph 5.6. its most notable characteristic was stable over a wide ph range (3-11 at 30 degrees c for 24 h) and a relatively high temperature (60 degrees c for 24 h). the k-m for pnpg was 0.338 mm and the v-max was 0.25 mmol nitrophenol/min/mg glucosidase at ph 5.6 and 50 degrees c. compared to its activity against pnpg (100%), the beta-glucosidase exhibited low levels of activity against other aryl-glycosides (less than 1%). additionally, the enzyme hydrolyzed the 20-c, beta-(1 -> 6)-glucoside of ginsenoside rb-1 to produce ginsenoside rd, but did not hydrolyze the other beta-(d)-glucosidic bonds of rb-1. the properties of the enzyme could make it become a useful tool in biotransformation of glucosides. (c) 2006 elsevier ltd. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; technology |
| 类目[WOS] | biochemistry & molecular biology ; biotechnology & applied microbiology ; engineering, chemical |
| 研究领域[WOS] | biochemistry & molecular biology ; biotechnology & applied microbiology ; engineering |
| 关键词[WOS] | thermophilic fungus ; rd ; saponins ; protein ; metabolism ; absorption ; excretion ; bacterium ; binding ; rats |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000239868300009 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/139952]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Dalian Med Univ, Dalian 116027, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luan, Hongwei,Liu, Xin,Qi, Xiaohui,et al. Purification and characterization of a novel stable ginsenoside Rb-1-hydrolyzing beta-D-glucosidase from China white jade snail[J]. process biochemistry,2006,41(9):1974-1980. |
| APA | Luan, Hongwei.,Liu, Xin.,Qi, Xiaohui.,Hu, Ying.,Hao, Dacheng.,...&Yang, Ling.(2006).Purification and characterization of a novel stable ginsenoside Rb-1-hydrolyzing beta-D-glucosidase from China white jade snail.process biochemistry,41(9),1974-1980. |
| MLA | Luan, Hongwei,et al."Purification and characterization of a novel stable ginsenoside Rb-1-hydrolyzing beta-D-glucosidase from China white jade snail".process biochemistry 41.9(2006):1974-1980. |
入库方式： OAI收割
来源：大连化学物理研究所
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