Preparation and application of cross-linked polyacrylamide coated column for capillary electrophoresis
文献类型:期刊论文
作者 | Tian, HZ; Yang, BC; Guan, YF |
刊名 | chemical journal of chinese universities-chinese
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出版日期 | 2006-02-10 |
卷号 | 27期号:2页码:218-221 |
关键词 | cross-liked polyacrylamide-coated column capillary electrophoresis protein separation alkaloid separation |
英文摘要 | a method was developed for the preparation of static coating onto the fused-silica columns with electroosmotic flow and prevent cross-linked polyacrylamide. the coated columns were effective to suppress the e the absorption of basic proteins on the column wall. the basic proteins were separated by using the coated columns at ph = 4.0, with retention time variation of less than 2.1% rsd. the average separation efficiencies were 2.4 x 10(5) plates/m for alkaloids. the cross-linked polyacrylamide coated columns can be prepared reproducibly by using this method. |
WOS标题词 | science & technology ; physical sciences |
类目[WOS] | chemistry, multidisciplinary |
研究领域[WOS] | chemistry |
关键词[WOS] | ephedrine ; performance ; separation ; proteins |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000235414300006 |
公开日期 | 2015-11-17 |
源URL | [http://159.226.238.44/handle/321008/139970] ![]() |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem & Micro Instrumentat, Dalian 116023, Peoples R China |
推荐引用方式 GB/T 7714 | Tian, HZ,Yang, BC,Guan, YF. Preparation and application of cross-linked polyacrylamide coated column for capillary electrophoresis[J]. chemical journal of chinese universities-chinese,2006,27(2):218-221. |
APA | Tian, HZ,Yang, BC,&Guan, YF.(2006).Preparation and application of cross-linked polyacrylamide coated column for capillary electrophoresis.chemical journal of chinese universities-chinese,27(2),218-221. |
MLA | Tian, HZ,et al."Preparation and application of cross-linked polyacrylamide coated column for capillary electrophoresis".chemical journal of chinese universities-chinese 27.2(2006):218-221. |
入库方式: OAI收割
来源:大连化学物理研究所
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