Coupling the immobilized trypsin microreactor of monolithic capillary with mu RPLC-MS/MS for shotgun proteome analysis
文献类型：期刊论文
| 作者 | Feng, S; Ye, ML; Jiang, XG; Jin, WH; Zou, HF |
| 刊名 | journal of proteome research
 |
| 出版日期 | 2006-02-01 |
| 卷号 | 5期号:2页码:422-428 |
| 关键词 | immobilized trypsin microreactor monolithic matrix shotgun proteomics mu RPLC-MS/MS |
| 英文摘要 | a nanoliter trypsin-based monolithic microreactor coupled with mu rplc-ms/ms was reported for shotgun proteome analysis. the proteins were rapidly digested by the microreactor, and the resulting protein digests were directly loaded onto a mu rplc column for separation followed with detection of the eluted peptides by tandem mass spectrometer. the digestion efficiency and stability of the microreactor was demonstrated by using bovine serum albumin as a model protein. when compared with an incubation time of more than 10 h by free trypsin in the conventional digestion approach, protein mixtures can be digested by the microreactor in several minutes. this system was applied to the analysis of the total cell lysate of saccharomyces cerevisiae. after a sequest database search, a total of 1578 unique peptides corresponding to 541 proteins were identified when 590 ng yeast protein was digested by the microreactor with an incubation time of only 1 min. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemical research methods |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | tandem mass-spectrometry ; solid-phase extraction ; liquid-chromatography ; affinity-chromatography ; enzymatic digestion ; yeast proteome ; gel-electrophoresis ; online ; identification ; proteins |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000235317600024 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140012]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Xinjiang Univ, Coll Chem & Chem Engn, Xinjiang 830046, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, S,Ye, ML,Jiang, XG,et al. Coupling the immobilized trypsin microreactor of monolithic capillary with mu RPLC-MS/MS for shotgun proteome analysis[J]. journal of proteome research,2006,5(2):422-428. |
| APA | Feng, S,Ye, ML,Jiang, XG,Jin, WH,&Zou, HF.(2006).Coupling the immobilized trypsin microreactor of monolithic capillary with mu RPLC-MS/MS for shotgun proteome analysis.journal of proteome research,5(2),422-428. |
| MLA | Feng, S,et al."Coupling the immobilized trypsin microreactor of monolithic capillary with mu RPLC-MS/MS for shotgun proteome analysis".journal of proteome research 5.2(2006):422-428. |
入库方式： OAI收割
来源：大连化学物理研究所
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