Quantitative analysis of fibrosis formation on the microcapsule surface with the use of picro-sirius red staining, polarized light microscopy, and digital image analysis
文献类型：期刊论文
| 作者 | Zhang, H; Sun, L; Wang, W; Ma, X |
| 刊名 | journal of biomedical materials research part a
 |
| 出版日期 | 2006 |
| 卷号 | 76a期号:1页码:120-125 |
| 关键词 | image analysis fibrosis collagen microcapsules polarized light microscopy stains and staining |
| 英文摘要 | transplantation of microencapsulated cells shows potential to treat a variety of diseases. to improve the quality of microcapsules, efficient methods need to be established and standardized for the evaluation of biocompatibility of microcapsules. to this end, a microscopical method was developed to analyze quantitatively the degree of fibrotic overgrowth (fo), which serves as the index of biocompatibility. in this method, sections were stained with picro-sirius red and illuminated with a polarized light microscope. the images were digitized with the use of a computer-video system in which collagenous content was depicted by gray levels i to 255. both the area of collagen and the thickness of fo were examined. the ratio of type i/type iii collagen and the thickness were used as indexes of the degree of fo. the data showed that the ratio of type i/type iii collagen and the thickness increased with time, and fo was not significant 4 weeks after transplantation. in comparison, this new method is more efficient to evaluate fo. (c) 2005 wiley periodicals, inc. |
| WOS标题词 | science & technology ; technology |
| 类目[WOS] | engineering, biomedical ; materials science, biomaterials |
| 研究领域[WOS] | engineering ; materials science |
| 关键词[WOS] | fibrotic overgrowth ; microencapsulated islets ; semiquantitative indexes ; liver fibrosis ; alginate ; collagen ; biocompatibility ; immunosuppression ; transplantation ; quantification |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000234105400014 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140030]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, H,Sun, L,Wang, W,et al. Quantitative analysis of fibrosis formation on the microcapsule surface with the use of picro-sirius red staining, polarized light microscopy, and digital image analysis[J]. journal of biomedical materials research part a,2006,76a(1):120-125. |
| APA | Zhang, H,Sun, L,Wang, W,&Ma, X.(2006).Quantitative analysis of fibrosis formation on the microcapsule surface with the use of picro-sirius red staining, polarized light microscopy, and digital image analysis.journal of biomedical materials research part a,76a(1),120-125. |
| MLA | Zhang, H,et al."Quantitative analysis of fibrosis formation on the microcapsule surface with the use of picro-sirius red staining, polarized light microscopy, and digital image analysis".journal of biomedical materials research part a 76a.1(2006):120-125. |
入库方式： OAI收割
来源：大连化学物理研究所
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