Methylation analysis of hMLH1 gene promoter by a bisulfate-sensitive single-strand conformation polymorphism-capillary electrophoresis method
文献类型：期刊论文
| 作者 | Shi, Xianzhe; Li, Jianhua; Zhao, Chunxia; Lv, Shen; Xu, Guowang |
| 刊名 | biomedical chromatography
 |
| 出版日期 | 2006-08-01 |
| 卷号 | 20期号:8页码:815-820 |
| 关键词 | DNA methylation hMLH1 promoter bisulfite-single strand conformation polymorphism capillary electrophoresis |
| 英文摘要 | dna methylation is an important epigenetic modification that alters transcription in those genes containing cpg islands. in this report, a novel dna methylation analysis method was developed employing bisulfite-single strand conformation polymorphism combined with capillary electrophoresis (bisulfite-sscp-ce). during the bisulfite treatment of genomic dna, a high concentration of sodium bisulfite (4.8 mol/l) was preferred in order to shorten reaction time and minimize template degradation. the methylated and unmethylated ssdna of hmlh1 promoter were simultaneously separated under the optimized ce conditions, including 6% slpa with 10% glycerol as sieving medium, 25 degrees c as separation temperature and 12 kv as running voltage. the heterogeneous methylation of hmlh1 promoter was identified in 13 of 64 colorectal cancer patients. moreover, hmlh1 promoter methylation had a significant relationship with protein expression loss and increased with the age of patients. our results indicated that dna methylation analysis for a large number of clinical samples would be facilitated by use of the bisulfite-sscp-ce method. copyright (c) 2005 john wiley & sons, ltd. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; biochemistry & molecular biology ; chemistry, analytical ; pharmacology & pharmacy |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry ; pharmacology & pharmacy |
| 关键词[WOS] | mutation detection ; cpg ; expression ; assay ; pcr |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000239541300020 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140042]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Dalian Med Univ, Secondary Affiliated Hosp, Dalian 116027, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Xianzhe,Li, Jianhua,Zhao, Chunxia,et al. Methylation analysis of hMLH1 gene promoter by a bisulfate-sensitive single-strand conformation polymorphism-capillary electrophoresis method[J]. biomedical chromatography,2006,20(8):815-820. |
| APA | Shi, Xianzhe,Li, Jianhua,Zhao, Chunxia,Lv, Shen,&Xu, Guowang.(2006).Methylation analysis of hMLH1 gene promoter by a bisulfate-sensitive single-strand conformation polymorphism-capillary electrophoresis method.biomedical chromatography,20(8),815-820. |
| MLA | Shi, Xianzhe,et al."Methylation analysis of hMLH1 gene promoter by a bisulfate-sensitive single-strand conformation polymorphism-capillary electrophoresis method".biomedical chromatography 20.8(2006):815-820. |
入库方式： OAI收割
来源：大连化学物理研究所
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