Homogeneous time-resolved fluoroimmunoassay of 3,5,3 '-triiodo-L-thyronine in human serum by using europium fluorescence energy transfer
文献类型：期刊论文
| 作者 | Wang, Guilan; Yuan, Jingli; Hai, Xiaodan; Matsumoto, Kazuko |
| 刊名 | talanta
 |
| 出版日期 | 2006-08-15 |
| 卷号 | 70期号:1页码:133-138 |
| 关键词 | homogeneous immunoassay 3 europium fluorescence resonance energy transfer 5 3 '-triiodo-L-thyronine |
| 英文摘要 | a sensitive homogeneous time-resolved fluoroimmunoassay method for 3,5,3'-triiodo-l-thyronine (t3) based on the fluorescence resonance energy transfer (fret) from a fluorescent eu3+ complex, {[(4,6-dichloro-1,3,5-triazin-2-yl)amino-biphenyl-4"'-yl]-2,2':6',2"-terpyridine-6,6"-diyl}bis(methylenenitrilo) tetrakis(acetate)-eu3+ (dtbta-eu3+) (lambda(ex,max)= 335 nm, lambda(em,max) = 615 nm), to an organic fluorescence dye cy5 has been developed. the new assay system combined the use of dtbta-eu3+-labeled t3-bovine serum albumin (bsa) conjugate and cy5-labeled anti-t3 monoclonal antibody for a competitive-type immunoassay. after the competitive reactions of dtbta-eu3+-labeled t3-bsa and t3 sample with cy5-labeled anti-t3 antibody, the t3 concentration was measured with a time-resolved mode by monitoring the sensitized emission of cy5 derived from fret in a homogeneous format. the method gives the detection limit of 0.26 ng/ml. the coefficient variations of the method are less than 2.0% and the recoveries are in the range of 80-111% for serum sample measurement. the concentrations of t3 in 30 human serum samples were determined. and the results were compared with those of the independently determined by a radio-immunoassay method. a good correlation was obtained with a correlation coefficient of 0.989. (c) 2005 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | terbium ; assay ; immunoassays ; triiodothyronine ; chelate ; markers ; labels ; tsh ; t3 |
| 收录类别 | ISTP ; SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000239709400024 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140090]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian 116023, Peoples R China 2.Waseda Univ, Dept Chem, Shinjuku Ku, Tokyo 1698555, Japan |
| 推荐引用方式 GB/T 7714 | Wang, Guilan,Yuan, Jingli,Hai, Xiaodan,et al. Homogeneous time-resolved fluoroimmunoassay of 3,5,3 '-triiodo-L-thyronine in human serum by using europium fluorescence energy transfer[J]. talanta,2006,70(1):133-138. |
| APA | Wang, Guilan,Yuan, Jingli,Hai, Xiaodan,&Matsumoto, Kazuko.(2006).Homogeneous time-resolved fluoroimmunoassay of 3,5,3 '-triiodo-L-thyronine in human serum by using europium fluorescence energy transfer.talanta,70(1),133-138. |
| MLA | Wang, Guilan,et al."Homogeneous time-resolved fluoroimmunoassay of 3,5,3 '-triiodo-L-thyronine in human serum by using europium fluorescence energy transfer".talanta 70.1(2006):133-138. |
入库方式： OAI收割
来源：大连化学物理研究所
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