A sheath-flow nanoelectrospray interface of microchip electrophoresis MS for glycoprotein and glycopeptide analysis
文献类型：期刊论文
| 作者 | Mao, Xiuli; Chu, Ivan K.; Lin, Bingcheng |
| 刊名 | electrophoresis
 |
| 出版日期 | 2006-12-01 |
| 卷号 | 27期号:24页码:5059-5067 |
| 关键词 | glycopeptides glycoprotein microchip electrophoresis MS nanoelectrospray interface |
| 英文摘要 | microchip was coupled with ms through a stable, sensitive, and controllable sheath-flow nanoelectrospray (nes) interface for glycoprotein and glycopeptide analysis. the nano-esi (nesi) was made with a delivery capillary, a commercial nes capillary, and a stainless steel (ss) tube which were connected together through a tee unit. high voltage for nes was applied on the ss tube and the commercial nes capillary was used as nes emitter. the delivery capillary was attached to the microchannel for delivering liquid from microchip to the nesi source. the flow rate of sheath liquid was optimized to be 100-200 nl/min which largely reduced the sample dilution. the detection limit of peptides on this microchip/ms platform was at femtomole level. glycoprotein and glycopeptides were also successfully analyzed on the platform. all the glycoforms and glycopeptides of ribonuclease b (rnase b) were identified with this method. some structures of the glycopeptides from rnase b were further characterized with ms/ms on the microchip, coupled with a quadrupole it-ms. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | electrospray mass-spectrometry ; on-a-chip ; microfluidic devices ; maldi-ms ; capillary ; separation ; performance ; emitters ; system |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000243304100022 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140134]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China 3.Chinese Acad Sci, Grad Sch, Dalian, Peoples R China 4.Univ Hong Kong, Genome Res Ctr, Hong Kong, Hong Kong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mao, Xiuli,Chu, Ivan K.,Lin, Bingcheng. A sheath-flow nanoelectrospray interface of microchip electrophoresis MS for glycoprotein and glycopeptide analysis[J]. electrophoresis,2006,27(24):5059-5067. |
| APA | Mao, Xiuli,Chu, Ivan K.,&Lin, Bingcheng.(2006).A sheath-flow nanoelectrospray interface of microchip electrophoresis MS for glycoprotein and glycopeptide analysis.electrophoresis,27(24),5059-5067. |
| MLA | Mao, Xiuli,et al."A sheath-flow nanoelectrospray interface of microchip electrophoresis MS for glycoprotein and glycopeptide analysis".electrophoresis 27.24(2006):5059-5067. |
入库方式： OAI收割
来源：大连化学物理研究所
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