A matrix of 3,4-diaminobenzophenone for the analysis of oligonucleotides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
文献类型：期刊论文
| 作者 | Yu Fu; Xu, Songyun; Pan, Chensong; Ye, Mingliang; Zou, Hanfa; Guo, Baochuan |
| 刊名 | nucleic acids research
 |
| 出版日期 | 2006 |
| 卷号 | 34期号:13 |
| 英文摘要 | a new matrix of 3,4-diaminobenzophenone (dabp) was demonstrated to be advantageous in the analysis of oligonucleotides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. with dabp as a matrix, intact oligonucleotide ions can be readily produced with lower laser powers, resulting in better detection limits, less fragmentation and fewer alkali metal ion adducts compared with the results obtained with conventional matrices. importantly, minimal fragmentation and fewer alkali metal ion adducts were seen even at low concentrations of oligonucleotides. it was also found that samples prepared with dabp are highly homogenous and therefore reducing the need for finding 'sweet' spots in maldi. in addition, excellent shot-to-shot reproducibility, resolution and signal-to-noise ratio were seen with dabp as the matrix. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemistry & molecular biology |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | uv-maldi ms ; cpg methylation ; nucleic-acids ; ion stability ; stranded-dna ; co-matrices ; desorption ; tof ; oligodeoxynucleotides ; additives |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000240583100005 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140135]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph R&A Ctr, Dalian 116023, Peoples R China 2.Cleveland State Univ, Dept Chem, Cleveland, OH 44115 USA |
| 推荐引用方式 GB/T 7714 | Yu Fu,Xu, Songyun,Pan, Chensong,et al. A matrix of 3,4-diaminobenzophenone for the analysis of oligonucleotides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry[J]. nucleic acids research,2006,34(13). |
| APA | Yu Fu,Xu, Songyun,Pan, Chensong,Ye, Mingliang,Zou, Hanfa,&Guo, Baochuan.(2006).A matrix of 3,4-diaminobenzophenone for the analysis of oligonucleotides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.nucleic acids research,34(13). |
| MLA | Yu Fu,et al."A matrix of 3,4-diaminobenzophenone for the analysis of oligonucleotides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry".nucleic acids research 34.13(2006). |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。