Role of carbonyl cyanide m-chlorophenylhydrazone in enhancing photobiological hydrogen production by marine green alga Platymonas subcordiformis
文献类型：期刊论文
| 作者 | Ran, CQ; Yu, XJ; Jin, MF; Zhang, W |
| 刊名 | biotechnology progress
 |
| 出版日期 | 2006-03-01 |
| 卷号 | 22期号:2页码:438-443 |
| 英文摘要 | we demonstrated that a significant volume of h-2 gas could be photobiologically produced by a marine green alga platymonas subcordiformis when an uncoupler of photophosphorylation, carbonyl cyanide in-chlorophenylhydrazone (cccp), was added after 32 h of anaerobic dark incubation, whereas a negligible volume of h-2 gas was produced without cccp. the role of cccp in enhancing photobiological h-2 production was delineated. cccp as an adry agent (agent accelerating the deactivation reactions of water-splitting enzyme system y) rapidly inhibited the photosystem h (psii) activity of p. subcordiformis cells, resulting in a markedly decline in the coupled oxygen evolution. the mitochondrial oxidative respiration was only slightly inactivated by cccp, which depleted o-2 in the light. as a result, anaerobiosis during the stage of photobiological h-2 evolution was established, preventing severe o-2 inactivation of the reversible hydrogenase in p. subcordiformis. the uncoupling effect of cccp accelerates electron transfer from water due to a disruption of the proton motive force and release of delta ph across the thylakoid membrane and thus enhances the accessibility of electron and h+ to hydrogenase. the electrons for hydrogen photoevolution are mainly from the photolysis of water (90%). upon the addition of cccp, chl a/b ratio increased, which implies a decrease in the light-harvesting psii antennae or an increase in psii/psi ratio, possibly resulting in higher efficiency of utilization of light energy. the enhancement of h-2 evolution by the addition of cccp is mostly due to the combination of the above three mechanisms. however, the disruption of the proton gradient across the thylakoid membrane may prevent a sustained photobiological h-2 evolution due to a shortfall of atp generation essential for the maintenance and repair functions of the cells. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology ; food science & technology |
| 研究领域[WOS] | biotechnology & applied microbiology ; food science & technology |
| 关键词[WOS] | photosynthetic electron-transport ; watersplitting enzyme-system ; chlamydomonas-reinhardtii ; deactivation reactions ; evolution ; photoproduction ; scenedesmus ; h-2 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000236783400015 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140142]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Bedford Pk, SA 5042, Australia |
| 推荐引用方式 GB/T 7714 | Ran, CQ,Yu, XJ,Jin, MF,et al. Role of carbonyl cyanide m-chlorophenylhydrazone in enhancing photobiological hydrogen production by marine green alga Platymonas subcordiformis[J]. biotechnology progress,2006,22(2):438-443. |
| APA | Ran, CQ,Yu, XJ,Jin, MF,&Zhang, W.(2006).Role of carbonyl cyanide m-chlorophenylhydrazone in enhancing photobiological hydrogen production by marine green alga Platymonas subcordiformis.biotechnology progress,22(2),438-443. |
| MLA | Ran, CQ,et al."Role of carbonyl cyanide m-chlorophenylhydrazone in enhancing photobiological hydrogen production by marine green alga Platymonas subcordiformis".biotechnology progress 22.2(2006):438-443. |
入库方式： OAI收割
来源：大连化学物理研究所
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