Fast extraction, amplification and analysis of genes from human blood
文献类型：期刊论文
| 作者 | Zhang, LH; Dang, FQ; Kaji, N; Baba, Y |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2006-02-17 |
| 卷号 | 1106期号:1-2页码:175-180 |
| 关键词 | gene analysis polymerase chain reaction microchip electrophoresis |
| 英文摘要 | in order to shorten the time spent on the sample preparation for gene analysis, a novel method was proposed through the combination of fast dna extraction and purification by generation capture disk, amplification by capillary polymerase chain reaction, and confirmation of amplification products by microchip electrophoresis. with this method, 3 mu l blood was enough to obtain adequate target fragments in human genes. under the optimal conditions in each step, the sample preparation for eight fragments in p-globin gene and four fragments in ras gene could be finished within 20 min. since all the experiments were performed on commercial instruments, this method showed a wide range of applicability. in addition, other advantages such as fast speed and low consumption of samples were demonstrated. all these merits proved that such a combination method was of great potential for the clinical diagnostics. (c) 2005 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | polymerase-chain-reaction ; capillary-electrophoresis ; sample preparation ; reaction products ; microchip electrophoresis ; pcr products ; dna analysis ; genomic dna ; polymorphisms ; population |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000235256800029 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140162]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Univ Tokushima, Grad Sch Pharmaceut Sci, CREST, JST,Dept Mol & Pharmaceut Biotechnol, Tokushima 7708505, Japan 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R & A Ctr, Dalian 116023, Peoples R China 3.Nagoya Univ, Grad Sch Engn, Dept Appl Chem, Chikusa Ku, Nagoya, Aichi 4648603, Japan 4.Furuno Elect Co Ltd, Nishinomiya, Hyogo 6628580, Japan 5.AIST, Hlth Technol Res Ctr, Takamatsu, Kagawa 7610395, Japan |
| 推荐引用方式 GB/T 7714 | Zhang, LH,Dang, FQ,Kaji, N,et al. Fast extraction, amplification and analysis of genes from human blood[J]. journal of chromatography a,2006,1106(1-2):175-180. |
| APA | Zhang, LH,Dang, FQ,Kaji, N,&Baba, Y.(2006).Fast extraction, amplification and analysis of genes from human blood.journal of chromatography a,1106(1-2),175-180. |
| MLA | Zhang, LH,et al."Fast extraction, amplification and analysis of genes from human blood".journal of chromatography a 1106.1-2(2006):175-180. |
入库方式： OAI收割
来源：大连化学物理研究所
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