Macroporous polyacrylamide-based monolithic column with immobilized pH gradient for protein analysis
文献类型：期刊论文
| 作者 | Zhu, G.; Zhang, L.; Liang, Z.; Zhang, W.; Zhang, Y. |
| 刊名 | molecular & cellular proteomics
 |
| 出版日期 | 2006-10-01 |
| 卷号 | 5期号:10页码:s141-s141 |
| 关键词 | capillary isoelectric focusing monolithic immobilized pH gradient polyacrylamide protein |
| 英文摘要 | monolithic materials were prepared in capillaries by in situ polymerization of acrylamide, glycidyl methacrylate, and n,n'-methylenebisacrylamide in the presence of 1,4-butanediol, dodecanol, and dmso as porogens. with ampholine attached to the surface of the porous monolith via epoxide groups, a monolithic-ipg (m-ipg) was formed and showed good mechanical and chemical stability. with such a column immobilized by ampholine 3.5-10, ief-mix 3.6-9.3 was separated and good linearity was obtained. the cief behavior of m-ipg was evinced by comparing the current with that in the open tubular capillary. in addition, the protein mixtures excreted from lung cancer cells of rats were analyzed with such a new m-ipg column. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemical research methods |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | performance |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000241506400323 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140192]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | CAS, Dalian Inst Chem Phys, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, G.,Zhang, L.,Liang, Z.,et al. Macroporous polyacrylamide-based monolithic column with immobilized pH gradient for protein analysis[J]. molecular & cellular proteomics,2006,5(10):s141-s141. |
| APA | Zhu, G.,Zhang, L.,Liang, Z.,Zhang, W.,&Zhang, Y..(2006).Macroporous polyacrylamide-based monolithic column with immobilized pH gradient for protein analysis.molecular & cellular proteomics,5(10),s141-s141. |
| MLA | Zhu, G.,et al."Macroporous polyacrylamide-based monolithic column with immobilized pH gradient for protein analysis".molecular & cellular proteomics 5.10(2006):s141-s141. |
入库方式： OAI收割
来源：大连化学物理研究所
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