Preparation and evaluation of C18-bonded 1-mu m silica particles for pressurized capillary electrochromatography
文献类型：期刊论文
| 作者 | Qu, Qishu; Lu, Xiao; Huang, Xiaojing; Hu, Xiaoya; Zhang, Yukui; Yan, Chao |
| 刊名 | electrophoresis
 |
| 出版日期 | 2006-10-01 |
| 卷号 | 27期号:20页码:3981-3987 |
| 关键词 | capillary column pressurized capillary electrochromatography silica |
| 英文摘要 | nonporous silica spheres (1 mu m) were synthesized and bonded with octadecylsilane functionality. these stationary phase particles were packed electrokinetically into fused-silica capillaries with 100 gm id for a length of 20 cm, which was evaluated by using pressurized cec (pcec). the efficiency of the c18 rp column was characterized through the theoretical plates of thiourea, benzyl alcohol, toluene, styrene, and naphthalene. the effects of experimental parameters such as the applied voltage, sample size, pump flow rate, ph value and the concentration of the buffer solution, and the content of methanol in the mobile phase, on-column efficiency were evaluated. column efficiency as high as 200 000 theoretical plates per meter for naphthalene was obtained with the optimal condition of 70% v/v methanol and 30% v/v of 10 mmol/l phosphate buffer (ph 7.8) at an applied voltage of 10 kv and a supplementary pressure of 500 psi. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | performance liquid-chromatography ; column technology ; separation ; efficiency ; electrophoresis ; peptides ; packing |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000241916100008 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140204]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Yangzhou Univ, Jiangsu Key Lab Environm Mat & Environm Engn, Coll Chem & Chem Engn, Yangzhou 225002, Jiangsu, Peoples R China 2.Unimicro Technol Inc, Pleasanton, CA USA 3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qu, Qishu,Lu, Xiao,Huang, Xiaojing,et al. Preparation and evaluation of C18-bonded 1-mu m silica particles for pressurized capillary electrochromatography[J]. electrophoresis,2006,27(20):3981-3987. |
| APA | Qu, Qishu,Lu, Xiao,Huang, Xiaojing,Hu, Xiaoya,Zhang, Yukui,&Yan, Chao.(2006).Preparation and evaluation of C18-bonded 1-mu m silica particles for pressurized capillary electrochromatography.electrophoresis,27(20),3981-3987. |
| MLA | Qu, Qishu,et al."Preparation and evaluation of C18-bonded 1-mu m silica particles for pressurized capillary electrochromatography".electrophoresis 27.20(2006):3981-3987. |
入库方式： OAI收割
来源：大连化学物理研究所
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