Synthesis and application of a nitroboronic acid-substituated silica for affinity chromatography
文献类型：期刊论文
| 作者 | Li Fanglou; Zhao Xinjie; Xu Guowang |
| 刊名 | chinese journal of analytical chemistry
 |
| 出版日期 | 2006-10-01 |
| 卷号 | 34期号:10页码:1366-1370 |
| 关键词 | affinity chromatography boronic acid affinity chromatography pre-column multidimensional high performance liquid chromatography direct injection |
| 英文摘要 | a boronic acid-substituted silica was synthesized by using a gamma-glycidoxypropyltrimethoxysilane as the spacer arm and using a 3-amino-4-nitrobenzeneboronic acid as the ligand. the 3-amino-4-nitrobenzeneboronic acid was modified from m-aminobenzeneboronic acid. then, boronic acid-substituted silica was packed in a stainless-steel column (35 mm x 4.6 mm i. d.) under the pressure of 2.07 mpa as an affinity pre-column. a coupled-column liquid chromatographic method for the direct analysis of 11 urinary nucleosides is described. efficient on-line clean-up and concentration of 11 nucleosides from urine samples were obtained by the affinity pre-column as the first column (extraction column) and an ods column (250 mm x 4.6 nun i. d.) as the second column (analytical column). the mobile phases applied consisted of 0.25 mol/l ammonium acetate (ph 7.95) on extraction column, and of 25 mmol/l potassium dihydrogen phosphate (ph 4.5) on analytical column, respectively. determination of urinary nucleosides was performed on analytical column by using a linear gradient elution comprising 25 mmol/l potassium dihydrogen phosphate (ph 4.5) and methanol-water (60:40, v/v) with uv detection at 260 nm. calibration plots of 11 standard nucleosides show good rsd, recovery and excellent linearity (r > 0. 995). |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000243502600002 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140211]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li Fanglou,Zhao Xinjie,Xu Guowang. Synthesis and application of a nitroboronic acid-substituated silica for affinity chromatography[J]. chinese journal of analytical chemistry,2006,34(10):1366-1370. |
| APA | Li Fanglou,Zhao Xinjie,&Xu Guowang.(2006).Synthesis and application of a nitroboronic acid-substituated silica for affinity chromatography.chinese journal of analytical chemistry,34(10),1366-1370. |
| MLA | Li Fanglou,et al."Synthesis and application of a nitroboronic acid-substituated silica for affinity chromatography".chinese journal of analytical chemistry 34.10(2006):1366-1370. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。