Studying protein-drug interaction by microfluidic chip affinity capillary electrophoresis with indirect laser-induced fluorescence detection
文献类型：期刊论文
| 作者 | Liu, Xiaojun; Liu, Xin; Liang, Aiye; Shen, Zheng; Zhang, Yu; Dai, Zhongpeng; Xiong, Bohui; Lin, Bingcheng |
| 刊名 | electrophoresis
 |
| 出版日期 | 2006-08-01 |
| 卷号 | 27期号:15页码:3125-3128 |
| 关键词 | binding constant indirect LIF microfluidic chip-affinity CE |
| 英文摘要 | we developed a microfluidic chip-affinity ce method based on indirect lif detection to study protein-drug interactions. the interaction between heparin and bsa was quantitatively studied, as a model system. in our method, sodium fluorescein was chosen as background, and redistilled water as marker to monitor eof the electrophoretic mobility changes of bsa were measured, with various concentrations of heparin added to the running buffer. each run was completed within 80 s. the binding constant was determined to be (1.24 +/- 0.05) x 10(3) m-1, which was in good agreement with that reported in the literature. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | fibroblast-growth-factor ; frontal analysis ; homogeneous immunoassay ; serum-albumin ; binding ; heparin ; microchip ; plasma ; tnt |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000239912600015 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140217]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116012, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Xiaojun,Liu, Xin,Liang, Aiye,et al. Studying protein-drug interaction by microfluidic chip affinity capillary electrophoresis with indirect laser-induced fluorescence detection[J]. electrophoresis,2006,27(15):3125-3128. |
| APA | Liu, Xiaojun.,Liu, Xin.,Liang, Aiye.,Shen, Zheng.,Zhang, Yu.,...&Lin, Bingcheng.(2006).Studying protein-drug interaction by microfluidic chip affinity capillary electrophoresis with indirect laser-induced fluorescence detection.electrophoresis,27(15),3125-3128. |
| MLA | Liu, Xiaojun,et al."Studying protein-drug interaction by microfluidic chip affinity capillary electrophoresis with indirect laser-induced fluorescence detection".electrophoresis 27.15(2006):3125-3128. |
入库方式： OAI收割
来源：大连化学物理研究所
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