Determination of organic acids in 1,3-propanediol fermentation broth by reversed phase high performance liquid chromatography
文献类型：期刊论文
| 作者 | Mou Ying; Wang Yuanhao; Xiu Zhilong; Wang Longxing; Xiao Hongbin |
| 刊名 | chinese journal of analytical chemistry
 |
| 出版日期 | 2006-09-01 |
| 卷号 | 34页码:s183-s186 |
| 关键词 | reversed phase high performance liquid chromatography organic acids 1 fermentation broth 3-propanediol |
| 英文摘要 | a reversed phase high performance liquid chromatographic method on a column of kromasil c-18 (5 mu m, 250 mm x 4. 6 mm i. d.) with an eluant containing 0.2% (v/v) phosphoric acid and acetonitrile (96. 5:3. 5, v/v) was used for the analysis of organic acids in 1,3-propanediol fermentation broth. the solution of samples was pretreated with chloroform. the formic acid, lactic acid, acetic acid, citric acid, succinic acid and fumaric acid were completely separated and determined in 8 minutes. the flow rate of mobile phase was 1.0 ml/min and the column temperature was 20 degrees c. the detection wavelength was 214 rim. the regression equation, the linear range, the correlation coefficient and detection limit of the standard curves were determined. the relative standard deviation (rsd, n = 5) was in the range of 1.0% - 2.4%, and the average recoveries of the method ranged from 97.7% to 100.5%. the method was proved to be a rapid, effective way to determine the organic acids in 1, 3-propanediol fermentation broth. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000244539600045 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140225]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Univ Technol, Dept Biosci & Biotechnol, Sch Environm & Biol Sci & Technol, Dalian 116024, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mou Ying,Wang Yuanhao,Xiu Zhilong,et al. Determination of organic acids in 1,3-propanediol fermentation broth by reversed phase high performance liquid chromatography[J]. chinese journal of analytical chemistry,2006,34:s183-s186. |
| APA | Mou Ying,Wang Yuanhao,Xiu Zhilong,Wang Longxing,&Xiao Hongbin.(2006).Determination of organic acids in 1,3-propanediol fermentation broth by reversed phase high performance liquid chromatography.chinese journal of analytical chemistry,34,s183-s186. |
| MLA | Mou Ying,et al."Determination of organic acids in 1,3-propanediol fermentation broth by reversed phase high performance liquid chromatography".chinese journal of analytical chemistry 34(2006):s183-s186. |
入库方式： OAI收割
来源：大连化学物理研究所
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