Liquid chromatography-mass spectrometric analysis of puerarin and its metabolite in human urine
文献类型：期刊论文
| 作者 | Tian, Hongzhe; Yang, Bingcheng; Xu, Jing; Wang, Hua; Guan, Yafeng |
| 刊名 | journal of separation science
 |
| 出版日期 | 2006-11-01 |
| 卷号 | 29期号:17页码:2692-2696 |
| 关键词 | capillary LC-MS daidzein isoflavonoid pueraria radix puerarin |
| 英文摘要 | a specific lg-ms method was developed that allowed simultaneous determination of puerarin (pu) and its major metabolite, daidzein (da), in human urine samples. pu and da were separated on a packed capillary ods column with on column concentration. identification and quantification of the analytes were performed with esi-q-tof mass spectroscopy in negative ionization mode. the method was validated, yielding calibration curves with correlation coefficients greater than 0.998. the loq for pu and da from human urine samples was 0.1 and 0.05 nmol/ml, respectively. assay accuracy and precision of quality control samples were within +/- 15%. recoveries of pu and da in spiked samples were in the range of 79.6-90.4 and 82.3-92.4%, respectively. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | time-resolved fluoroimmunoassay ; electrochemical detection ; capillary-electrophoresis ; quantitative-analysis ; daidzein ; genistein ; isoflavones ; performance ; plasma ; soy |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000243338600017 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140270]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Dept Instrumentat & Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tian, Hongzhe,Yang, Bingcheng,Xu, Jing,et al. Liquid chromatography-mass spectrometric analysis of puerarin and its metabolite in human urine[J]. journal of separation science,2006,29(17):2692-2696. |
| APA | Tian, Hongzhe,Yang, Bingcheng,Xu, Jing,Wang, Hua,&Guan, Yafeng.(2006).Liquid chromatography-mass spectrometric analysis of puerarin and its metabolite in human urine.journal of separation science,29(17),2692-2696. |
| MLA | Tian, Hongzhe,et al."Liquid chromatography-mass spectrometric analysis of puerarin and its metabolite in human urine".journal of separation science 29.17(2006):2692-2696. |
入库方式： OAI收割
来源：大连化学物理研究所
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