Optimization of recombinant cytochrome p450 2C9 protein production in Escherichia coli DH5 alpha by statistically-based experimental design
文献类型：期刊论文
| 作者 | Hao, Da C.; Zhu, Pan H.; Yang, Sheng L.; Yang, Ling |
| 刊名 | world journal of microbiology & biotechnology
 |
| 出版日期 | 2006-11-01 |
| 卷号 | 22期号:11页码:1169-1176 |
| 关键词 | cytochrome P4502C9 heterologous expression optimization Taguchi orthogonal design desirability function central composite design |
| 英文摘要 | cytochrome p450 (cyp) 2c9 is of major importance in drug metabolism. however, the low yield of recombinant cyp2c9 protein in e. coli strains prevents its extensive use in the study of in vitro drug metabolism. in the present study, taguchi design and desirability function were first used to investigate the effect of medium components (glycerol, delta-ala, iptg, ampicillin, chloramphenicol, inoculum density, peptone, thiamine, trace elements, nh4cl, and mgso4) on recombinant human cyp2c9 production by e. coli dh5 alpha. an l-12 (2(11)) orthogonal array was used to design the experiments to screen out the most influential factors. the cyp concentration and the specific content of cyp were considered as two product quality variables. a desirability function was applied to combine these two qualities as a single objective function. optimization via central composite design (ccd) was then undertaken to yield the best performance. the confirmation experiments indicated that the expression performance under the optimized conditions was better than those obtained under other conditions. a compromise between conflicting goals, such as achievement of good yield of recombinant cyp2c9 and facility of the following purification, was found by means of the desirability function d. this is the first report that combined taguchi design and ccd, and performed experiments in a multiresponse framework to optimize the production of human cyp in a recombinant e. coli strain. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | response-surface methodology ; fermentation conditions ; desirability function ; medium constituents ; ethanol ; resolution ; expression ; cyp2c9 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000241905000009 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140272]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Shanghai Res Ctr Biotechnol, Shanghai, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hao, Da C.,Zhu, Pan H.,Yang, Sheng L.,et al. Optimization of recombinant cytochrome p450 2C9 protein production in Escherichia coli DH5 alpha by statistically-based experimental design[J]. world journal of microbiology & biotechnology,2006,22(11):1169-1176. |
| APA | Hao, Da C.,Zhu, Pan H.,Yang, Sheng L.,&Yang, Ling.(2006).Optimization of recombinant cytochrome p450 2C9 protein production in Escherichia coli DH5 alpha by statistically-based experimental design.world journal of microbiology & biotechnology,22(11),1169-1176. |
| MLA | Hao, Da C.,et al."Optimization of recombinant cytochrome p450 2C9 protein production in Escherichia coli DH5 alpha by statistically-based experimental design".world journal of microbiology & biotechnology 22.11(2006):1169-1176. |
入库方式： OAI收割
来源：大连化学物理研究所
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