Rapid protein digestion and identification using monolithic enzymatic microreactor coupled with nano-liquid chromatography-electro spray ionization mass spectrometry
文献类型：期刊论文
| 作者 | Duan, JC; Sun, LL; Liang, Z; Zhang, J; Wang, H; Zhang, LH; Zhang, WB; Zhang, YK |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2006-02-17 |
| 卷号 | 1106期号:1-2页码:165-174 |
| 关键词 | monolithic column enzymatic reactor protein identification nano-liquid chromatography-tandem mass spectrometry |
| 英文摘要 | a novel monolithic enzymatic microreactor was prepared in the fused-silica capillary by in situ polymerization of acrylamide (aa), nacryloxysuccinimide (nas) and ethylene dimethacrylate (edma) in the presence of a binary porogenic mixture of dodecanol and cyclohexanol, which could offer very low back pressure, enabling the fast digestion of proteins. the performance of the monolithic microreactor was demonstrated by digesting cytochrome c at high flow rate, and the comparisons between the in-solution digestion and on-column reaction were made by a nano-high performance liquid chromatography-mass spectrometry (nano-fiplc-ms) system. the performance of the monolithic microreactor was demonostrated with the digestion of cytochrome c at the fast flow rate of 1 mu l-min, which afforded a residence time of 7 s, yielding a sequence coverage of 54.81% using strict multiple database searching thresholds. furture more, a mixture of four standard proteins was digested and analyzed using the on-line digestion and nano-hplc-ms system. the results showed the promising of such a system in the analysis of protein mixture. (c) 2005 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | porous polymer monoliths ; capillary-electrophoresis ; proteolytic digestion ; trypsin microreactor ; peptide separation ; stationary phases ; sol-gel ; online ; proteomics ; chip |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000235256800028 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140278]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Duan, JC,Sun, LL,Liang, Z,et al. Rapid protein digestion and identification using monolithic enzymatic microreactor coupled with nano-liquid chromatography-electro spray ionization mass spectrometry[J]. journal of chromatography a,2006,1106(1-2):165-174. |
| APA | Duan, JC.,Sun, LL.,Liang, Z.,Zhang, J.,Wang, H.,...&Zhang, YK.(2006).Rapid protein digestion and identification using monolithic enzymatic microreactor coupled with nano-liquid chromatography-electro spray ionization mass spectrometry.journal of chromatography a,1106(1-2),165-174. |
| MLA | Duan, JC,et al."Rapid protein digestion and identification using monolithic enzymatic microreactor coupled with nano-liquid chromatography-electro spray ionization mass spectrometry".journal of chromatography a 1106.1-2(2006):165-174. |
入库方式： OAI收割
来源：大连化学物理研究所
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