Capillary electrochromatography with zwitterionic stationary phase on the lysine-bonded poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolithic capillary column
文献类型：期刊论文
| 作者 | Dong, Xiaoli; Dong, Jing; Ou, Junjie; Zhu, Yan; Zou, Hanfa |
| 刊名 | electrophoresis
 |
| 出版日期 | 2006-06-01 |
| 卷号 | 27期号:12页码:2518-2525 |
| 关键词 | aromatic acids basic pharmaceuticals capillary electrochromatography lysine-boncled zwitterion methacrylate-based monolith |
| 英文摘要 | a polymer-based neutral monolithic capillary column was prepared by radical polymerization of glycidyl methacrylate and ethylene dimethacrylate in a 100 lam id fused-silica capillary, and the prepared monolithic column was subsequently modified based on a ring opening reaction of epoxide groups with 1 m lysine in solution (ph 8.0) at 75 degrees c for 10 h to produce a lysine chemically bonded stationary phases in capillary column. the ring opening reaction conditions were optimized so that the column could generate substantial eof due to the zwitterionic functional groups of the lysine covalently bonded on the polymer monolithic rod, the prepared column can generate cathodic and anodic eof by varying the ph values of running buffer during cec separation. eof reached the maximum of -2.0 x 10(-8) m(2)v(-1)s(-1) and 2.6 x 10(-8) m(2)v(-1)s(-1) with ph of the running buffer of 2.25 and 10, respectively. as a consequence, neutral compounds, ionic solutes such as phenols, aromatic acids, anilines, and basic pharmaceuticals were all successfully separated on the column by cec. hydrophobic interaction is responsible for separation of neutral analytes. in addition, the electrostatic and hydrophobic interaction and the electrophoretic migration play a significant role in separation of the ionic or ionizable analytes. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | liquid-chromatography ; silica column ; amino-acids ; separation ; exchange ; electrophoresis ; constants ; driven ; flow |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000238899400025 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140280]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China 2.Zhejiang Univ, Dept Chem, Hangzhou 310027, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dong, Xiaoli,Dong, Jing,Ou, Junjie,et al. Capillary electrochromatography with zwitterionic stationary phase on the lysine-bonded poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolithic capillary column[J]. electrophoresis,2006,27(12):2518-2525. |
| APA | Dong, Xiaoli,Dong, Jing,Ou, Junjie,Zhu, Yan,&Zou, Hanfa.(2006).Capillary electrochromatography with zwitterionic stationary phase on the lysine-bonded poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolithic capillary column.electrophoresis,27(12),2518-2525. |
| MLA | Dong, Xiaoli,et al."Capillary electrochromatography with zwitterionic stationary phase on the lysine-bonded poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolithic capillary column".electrophoresis 27.12(2006):2518-2525. |
入库方式： OAI收割
来源：大连化学物理研究所
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