Preparation of protein-loaded microspheres with size <= 10 mu m by electrostatic droplet generation technology
文献类型：期刊论文
| 作者 | Xue, WM; Liu, XD; Yu, WT; Ma, XJ |
| 刊名 | chinese science bulletin
 |
| 出版日期 | 2006-02-01 |
| 卷号 | 51期号:3页码:279-286 |
| 关键词 | electrostatic droplet generation technology insulin hemoglobin alginate microsphere size encapsulation efficiency |
| 英文摘要 | the development of non-injection route for protein drugs, especially oral administration, has been the main focus of controlled release of drugs. to overcome obstacles unsolved such as enzyme degradation and penetration barrier of intestinal epithelium, technologies using microspheres as carrier of protein drugs have been proven potential to realize oral administration. it has been demonstrated that microspheres can not only protect proteins, but also facilitate the penetration and absorption through peyer's patches when the size is smaller than 10 mu m. therefore, the objective of this paper is to prepare protein-loaded microspheres with size <= 10 mu m. electrostatic droplet generation technology was used with insulin and hemoglobin as drug models and sodium alginate as microsphere material. by decreasing the surface tension of feed solution by adding surfactant, and improving electric field distribution by changing the shape of container and electrode for gelation solution, protein-loaded microspheres with mean size less than 10 mu m were successfully produced through needle with diameter of 400 pm. the microspheres showed good sphericity and narrow size distribution. the mean standard variance of size distribution was 1.61. the encapsulation efficiency of proteins was over 70%. moreover, the significance analysis of factors influencing the size of protein loaded microspheres was carried out through orthogonal experiments, which showed that output voltage (u), needle diameter (d) and the distance between needle tips to the surface of gelation solution (delta) influenced significantly the size of micro-spheres. finally, the statistic analysis showed that when confidence level was alpha=0.05, and alpha=0.1, confidence interval of microsphere size can be (6.2545, 10.1735) and (6.6022, 9.8258) correspondingly, suggesting that there is good repeatability and reliability for improving electrostatic droplet generation technology to prepare protein-loaded microspheres with size <= 10 mu m. |
| WOS标题词 | science & technology |
| 类目[WOS] | multidisciplinary sciences |
| 研究领域[WOS] | science & technology - other topics |
| 关键词[WOS] | oral delivery ; microbeads ; peptide ; drugs |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000236651200004 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140290]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.NW Univ Xian, Coll Chem Engn, Xian 710069, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xue, WM,Liu, XD,Yu, WT,et al. Preparation of protein-loaded microspheres with size <= 10 mu m by electrostatic droplet generation technology[J]. chinese science bulletin,2006,51(3):279-286. |
| APA | Xue, WM,Liu, XD,Yu, WT,&Ma, XJ.(2006).Preparation of protein-loaded microspheres with size <= 10 mu m by electrostatic droplet generation technology.chinese science bulletin,51(3),279-286. |
| MLA | Xue, WM,et al."Preparation of protein-loaded microspheres with size <= 10 mu m by electrostatic droplet generation technology".chinese science bulletin 51.3(2006):279-286. |
入库方式： OAI收割
来源：大连化学物理研究所
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