Proliferation, viability, and metabolism of human tumor and normal cells cultured in microcapsule
文献类型：期刊论文
| 作者 | Zhang, Xulang; Wang, Wei; Xie, Yubing; Zhang, Ying; Wang, Xiuli; Guo, Xin; Ma, Xiaojun |
| 刊名 | applied biochemistry and biotechnology
 |
| 出版日期 | 2006-07-01 |
| 卷号 | 134期号:1页码:61-76 |
| 关键词 | alginate microcapsule cell encapsulation poly-L-lysine tumor cell |
| 英文摘要 | in this study, we investigated the effect of the microenvironment provided by alginate-poly-l-lysine-alginate (apa) microcapsule with liquefied or gelled core on the proliferation, viability, and metabolism of human cells, including anchorage-dependent mcf-7 breast cancer cells and primary fibroblasts, and anchorage-independent k-562 leukemia cells; cells in conventional culture were used as control. the growth pattern of cells in microcapsule was examined by phase-contrast micrography. the cell viability, proliferation, organization, and gene expression were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, hematoxylin and eosin staining, live/dead staining, 5-bromo-20-deoxyuridine labeling, and immunohistochemistry, respectively. cell metabolism was determined by measuring glucose and lactate concentrations in medium. the results demonstrate that apa microcapsule with liquefied core provides a microenvironment for both anchorage-dependent and anchorage-independent cells to grow into a large cell aggregate and maintain cell viability at a constant level for a period of time. in conclusion, cells in apa microcapsule are alive and have proliferation potential with lower metabolism rate. apa microcapsule may be a useful tool for in vitro tumor cell modeling and anticancer drug screening as well as for cancer gene therapy. in addition, it lays a solid foundation for the use of microencapsulation in cell culture in vitro and cell implantation in vivo. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemistry & molecular biology ; biotechnology & applied microbiology |
| 研究领域[WOS] | biochemistry & molecular biology ; biotechnology & applied microbiology |
| 关键词[WOS] | in-vitro ; microencapsulation ; therapy ; islets |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000239041300006 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140321]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Xulang,Wang, Wei,Xie, Yubing,et al. Proliferation, viability, and metabolism of human tumor and normal cells cultured in microcapsule[J]. applied biochemistry and biotechnology,2006,134(1):61-76. |
| APA | Zhang, Xulang.,Wang, Wei.,Xie, Yubing.,Zhang, Ying.,Wang, Xiuli.,...&Ma, Xiaojun.(2006).Proliferation, viability, and metabolism of human tumor and normal cells cultured in microcapsule.applied biochemistry and biotechnology,134(1),61-76. |
| MLA | Zhang, Xulang,et al."Proliferation, viability, and metabolism of human tumor and normal cells cultured in microcapsule".applied biochemistry and biotechnology 134.1(2006):61-76. |
入库方式： OAI收割
来源：大连化学物理研究所
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