中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Investigations of the interaction between 3-aminophenylboronic acid and bovine serum albumin using fluorimetry

文献类型:期刊论文

作者Wang Hua-Fang; Li Wen-You; He Xi-Wen; Chen Lang-Xing; Zhang Yu-Kui
刊名acta chimica sinica
出版日期2007-01-14
卷号65期号:1页码:43-48
关键词3-aminophenylboronic acid bovine serum albumin fluorescence quenching molecularly imprinting
英文摘要in order to understand the chemical mechanism and optimal conditions of the molecular imprinting, the interaction between 3-aminophenylboronic acid (apba) and bovine serum albumin (bsa) was investigated using fluorescence quenching method with many factors, as the ph value, ionic strength and others, which maybe influence the interaction. the research results indicate that under appropriate ionic strength and ph 6.25, the fluorescence quenching shows that apba strongly bound with the tryptophan of bsa, and the complex was formed with a molar ratio of 2 : 1. their apparent binding constant is k-a = 1.0 x 10(11) l-2.mol(-2), so their chemical bindings between donor bsa and acceptor apba are relatively strong. according to the results, the interaction mechanism between apba and bsa is predicted. during following research, it will be entirely possible to separate or enrich bsa composition, maybe it could improve imprinting and eluting efficiency greatly.
WOS标题词science & technology ; physical sciences
类目[WOS]chemistry, multidisciplinary
研究领域[WOS]chemistry
关键词[WOS]molecularly imprinted polymers ; binding ; surface
收录类别SCI
语种英语
WOS记录号WOS:000243606300008
公开日期2015-11-17
源URL[http://159.226.238.44/handle/321008/140405]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
作者单位1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Acad Sinica, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
推荐引用方式
GB/T 7714
Wang Hua-Fang,Li Wen-You,He Xi-Wen,et al. Investigations of the interaction between 3-aminophenylboronic acid and bovine serum albumin using fluorimetry[J]. acta chimica sinica,2007,65(1):43-48.
APA Wang Hua-Fang,Li Wen-You,He Xi-Wen,Chen Lang-Xing,&Zhang Yu-Kui.(2007).Investigations of the interaction between 3-aminophenylboronic acid and bovine serum albumin using fluorimetry.acta chimica sinica,65(1),43-48.
MLA Wang Hua-Fang,et al."Investigations of the interaction between 3-aminophenylboronic acid and bovine serum albumin using fluorimetry".acta chimica sinica 65.1(2007):43-48.

入库方式: OAI收割

来源:大连化学物理研究所

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