Investigations of the interaction between 3-aminophenylboronic acid and bovine serum albumin using fluorimetry
文献类型:期刊论文
作者 | Wang Hua-Fang; Li Wen-You; He Xi-Wen; Chen Lang-Xing; Zhang Yu-Kui |
刊名 | acta chimica sinica
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出版日期 | 2007-01-14 |
卷号 | 65期号:1页码:43-48 |
关键词 | 3-aminophenylboronic acid bovine serum albumin fluorescence quenching molecularly imprinting |
英文摘要 | in order to understand the chemical mechanism and optimal conditions of the molecular imprinting, the interaction between 3-aminophenylboronic acid (apba) and bovine serum albumin (bsa) was investigated using fluorescence quenching method with many factors, as the ph value, ionic strength and others, which maybe influence the interaction. the research results indicate that under appropriate ionic strength and ph 6.25, the fluorescence quenching shows that apba strongly bound with the tryptophan of bsa, and the complex was formed with a molar ratio of 2 : 1. their apparent binding constant is k-a = 1.0 x 10(11) l-2.mol(-2), so their chemical bindings between donor bsa and acceptor apba are relatively strong. according to the results, the interaction mechanism between apba and bsa is predicted. during following research, it will be entirely possible to separate or enrich bsa composition, maybe it could improve imprinting and eluting efficiency greatly. |
WOS标题词 | science & technology ; physical sciences |
类目[WOS] | chemistry, multidisciplinary |
研究领域[WOS] | chemistry |
关键词[WOS] | molecularly imprinted polymers ; binding ; surface |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000243606300008 |
公开日期 | 2015-11-17 |
源URL | [http://159.226.238.44/handle/321008/140405] ![]() |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
作者单位 | 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China 2.Acad Sinica, Dalian Inst Chem Phys, Dalian 116011, Peoples R China |
推荐引用方式 GB/T 7714 | Wang Hua-Fang,Li Wen-You,He Xi-Wen,et al. Investigations of the interaction between 3-aminophenylboronic acid and bovine serum albumin using fluorimetry[J]. acta chimica sinica,2007,65(1):43-48. |
APA | Wang Hua-Fang,Li Wen-You,He Xi-Wen,Chen Lang-Xing,&Zhang Yu-Kui.(2007).Investigations of the interaction between 3-aminophenylboronic acid and bovine serum albumin using fluorimetry.acta chimica sinica,65(1),43-48. |
MLA | Wang Hua-Fang,et al."Investigations of the interaction between 3-aminophenylboronic acid and bovine serum albumin using fluorimetry".acta chimica sinica 65.1(2007):43-48. |
入库方式: OAI收割
来源:大连化学物理研究所
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