Capillary hydrophilic interaction chromatography/mass spectrometry for simultaneous determination of multiple neurotransmitters in primate cerebral cortex
文献类型：期刊论文
| 作者 | Zhang, Xiaozhe; Rauch, Alexander; Lee, Han; Xiao, Hongbin; Rainer, Gregor; Logothetis, Nikos K. |
| 刊名 | rapid communications in mass spectrometry
 |
| 出版日期 | 2007 |
| 卷号 | 21期号:22页码:3621-3628 |
| 英文摘要 | a diverse array of neurotransmitters and neuromodulators control and affect brain function. a profound understanding of the signaling pathways and the neural circuits underlying behavior is therefore likely to require the tracking of concentration changes of active neurochemicals. in the present study, we demonstrate the feasibility of a method allowing the simultaneous determination of the concentrations of six neurotransmitters: acetylcholine, serotonin, dopamine, gamma-aminobutyric acid (gaba), glutamate and aspartate, in the extracellular brain fluid (ebf). we used hydrophilic interaction chromatography (hilic) coupled to tandem mass spectrometry (ms/ms) to analyze the ebf from the monkey brain. a push-pull sampling method was used to collect ebf from the prefrontal cortex (pfc) of conscious monkeys at flow rates in the range of low nl/min. the detection limits of acetylcholine, serotonin, dopamine, gaba, glutamate and aspartate were 0.015,0.15,0.3,1.2, 6 and 15 femtomoles, respectively, allowing us to quantitatively determine the concentrations of these six neurotransmitters simultaneously from 500 nl in vivo samples. we conclude that hilic/ms/ms combined with the push-pull sampling method represents a sensitive technique for simultaneous monitoring of neurotransmitters from ebf samples. copyright (c) 2007 john wiley & sons, ltd. |
| WOS标题词 | science & technology ; physical sciences ; technology |
| 类目[WOS] | chemistry, analytical ; spectroscopy |
| 研究领域[WOS] | chemistry ; spectroscopy |
| 关键词[WOS] | amino-acid neurotransmitters ; ionization-mass-spectrometry ; gamma-aminobutyric-acid ; in-vivo microdialysis ; push-pull perfusion ; electrospray-ionization ; liquid-chromatography ; polar compounds ; acetylcholine ; online |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000250993100011 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140425]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Max Planck Inst Biol Cybernet, D-72076 Tubingen, Germany 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Xiaozhe,Rauch, Alexander,Lee, Han,et al. Capillary hydrophilic interaction chromatography/mass spectrometry for simultaneous determination of multiple neurotransmitters in primate cerebral cortex[J]. rapid communications in mass spectrometry,2007,21(22):3621-3628. |
| APA | Zhang, Xiaozhe,Rauch, Alexander,Lee, Han,Xiao, Hongbin,Rainer, Gregor,&Logothetis, Nikos K..(2007).Capillary hydrophilic interaction chromatography/mass spectrometry for simultaneous determination of multiple neurotransmitters in primate cerebral cortex.rapid communications in mass spectrometry,21(22),3621-3628. |
| MLA | Zhang, Xiaozhe,et al."Capillary hydrophilic interaction chromatography/mass spectrometry for simultaneous determination of multiple neurotransmitters in primate cerebral cortex".rapid communications in mass spectrometry 21.22(2007):3621-3628. |
入库方式： OAI收割
来源：大连化学物理研究所
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