Ouantification of cis-abienol in oriental tobacco leaves by LC
文献类型：期刊论文
| 作者 | Ding, Li; Xie, Fuwei; Zhao, Mingyue; Xie, Jianping; Xu, Guowang |
| 刊名 | chromatographia
 |
| 出版日期 | 2007-10-01 |
| 卷号 | 66期号:7-8页码:529-532 |
| 关键词 | liquid chromatography cis-Abienol oriental tobacco |
| 英文摘要 | a rapid and accurate method for the quantification of cis-abienol in oriental tobacco leaves by normal phase liquid chromatography was developed. freeze-dried tobacco samples were sonicated in methylene chloride for 10 min. the supernatant was purified using a silica gel solid phase extraction cartridge. ten milliliter of the resulting methylene chloride eluate was collected, then separated on a 250 x 4.6 mm, 5 pm particle-size cn column with n-hexane: ethyl acetate, 100:2 (v/v) at a flow rate of 1 ml min(-1). cis-abienol was detected by uv absorption at 254 nm. the linear range was from 2.14 x 10(-4) to 4.28 x 10(-2) mg ml(-1) and the correlation coefficient was 1.000. the average recovery was 98.7, 105.2 and 103.1% in five replicated sets of tobacco samples spiked with 0.2856, 0.7140 and 1.904 mg cis-abienol. the relative standard deviations (rsds) were 1.04, 0.63 and 1.25%, respectively (n = 5). limit of detection (s/n = 3) was 21.84 pg g(-1) and limit of quantification (s/n = 10) was 72.80 mu g g(-1). the method was found to be suitable for determination of cis-abienol in oriental tobacco leaves. furthermore, pure cis-abienol used for method validation was obtained by preparative reversed phase high -performance liquid chromatography. identification was performed by uv detection, nuclear magnetic resonance and mass spectrometry. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | chemistry ; leaf |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000250366300012 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140500]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.CNTC, Zhengzhou Tobacco Res Inst, Dept Chem, Zhengzhou 450001, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Li,Xie, Fuwei,Zhao, Mingyue,et al. Ouantification of cis-abienol in oriental tobacco leaves by LC[J]. chromatographia,2007,66(7-8):529-532. |
| APA | Ding, Li,Xie, Fuwei,Zhao, Mingyue,Xie, Jianping,&Xu, Guowang.(2007).Ouantification of cis-abienol in oriental tobacco leaves by LC.chromatographia,66(7-8),529-532. |
| MLA | Ding, Li,et al."Ouantification of cis-abienol in oriental tobacco leaves by LC".chromatographia 66.7-8(2007):529-532. |
入库方式： OAI收割
来源：大连化学物理研究所
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