Cooperative effects in the fluorescent labeling of proteins studied by high-performance liquid chromatography
文献类型：期刊论文
| 作者 | Qiao Xiao-Qiang; Zhang Lin; Liang Zhen; Zhang Wei-Bing; Zhang Li-Hua; Zhang Yu-Kui |
| 刊名 | chemical journal of chinese universities-chinese
 |
| 出版日期 | 2007-09-01 |
| 卷号 | 28期号:9页码:1657-1659 |
| 关键词 | fluorescent labeling protein cooperative effect high-performance liquid chromatography (HPLC) |
| 英文摘要 | to improve the detection sensitivity of low abundance proteins in samples, fluorescence labeling might be a good solution method. in this manuscript, with 5-( {2-[ (iodoacetyl) amino] ethyl} amino) naphthalene-1-sulfonic acid (1,5-i-aedans) as the derivatizing reagent, the cooperative effects of insulin and bsa during the derivatization were studied. through hplc analysis, it was found that with,bsa added, the detection sensitivity of the derivatized insulin could be improved. when the concentration ratio of bsa to insulin reached 200: 1, the peak area of insulin was increased by over 4-fold, which demonstrates that the existence of bsa had a positive effect on the derivatization of insulin. furthermore, even when the ratio reached 1000: 1, the sin of insulin was 8, which could not be detected without bsa being added. however, on the contrary, with insulin being added, the peak area of the derivatized bsa was decreased, demonstrating that insulin had a negative effect on that of bsa. all these results might be of great significance to the detection of low abundance proteins in proteome study. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | molecules ; peptides ; binding |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000249629600013 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140501]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qiao Xiao-Qiang,Zhang Lin,Liang Zhen,et al. Cooperative effects in the fluorescent labeling of proteins studied by high-performance liquid chromatography[J]. chemical journal of chinese universities-chinese,2007,28(9):1657-1659. |
| APA | Qiao Xiao-Qiang,Zhang Lin,Liang Zhen,Zhang Wei-Bing,Zhang Li-Hua,&Zhang Yu-Kui.(2007).Cooperative effects in the fluorescent labeling of proteins studied by high-performance liquid chromatography.chemical journal of chinese universities-chinese,28(9),1657-1659. |
| MLA | Qiao Xiao-Qiang,et al."Cooperative effects in the fluorescent labeling of proteins studied by high-performance liquid chromatography".chemical journal of chinese universities-chinese 28.9(2007):1657-1659. |
入库方式： OAI收割
来源：大连化学物理研究所
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