Spectroscopy study on the interaction of colchicine and human serum albumin
文献类型：期刊论文
| 作者 | Ma Jun-Yan2; Chen Ke-Hai2; Zheng Xue-Fang1,2; Guo Ming1; Ma Jing2; Tang Qian2; Wang Yu-Lian2; Hu Jie-Han1,3 |
| 刊名 | spectroscopy and spectral analysis
 |
| 出版日期 | 2007-12-01 |
| 卷号 | 27期号:12页码:2485-2489 |
| 关键词 | colchicine human serum albumin UV-Vis spectra fluorescence spectra circular dichroism spectra |
| 英文摘要 | the binding reaction of colchicine with human serum albumin(hsa) was studied by uv-vis absorption, fluorescence and circular dichroism. spectrometry. the results indicated that colchicine led to the increase in uv absorption and the quenching of intrinsic fluorescence of hsa. as the temperature increased, the quenching constant k-sv decreased. the binding constants and the numbers of the binding sites of the interaction between colchicine and hsa at different temperatures were obtained. the thermodynamic parameters, enthalpy change (delta h) and entropy change (delta s), were calculated to be - 11. 66 kj . mol(-1) and 51. 507 j (mol . k)(-1) respectively according to van't hoff equation, which suggested that the main binding force between colchicine and hsa was static interaction. the protein conformation was altered(cd date) with decreasing of a-helices in the presence of colchicine. the results showed that the quenching mechanism of the combination of colchicine with human serum albumin was a static quenching procedure. |
| WOS标题词 | science & technology ; technology |
| 类目[WOS] | spectroscopy |
| 研究领域[WOS] | spectroscopy |
| 关键词[WOS] | fluorescence ; mechanism ; binding |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000253064500026 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140519]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Univ, Liaoning Key Lab Bioorgan Chem, Dalian 116622, Peoples R China 2.Dalian Univ, Coll Bioengn, Dalian 116622, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma Jun-Yan,Chen Ke-Hai,Zheng Xue-Fang,et al. Spectroscopy study on the interaction of colchicine and human serum albumin[J]. spectroscopy and spectral analysis,2007,27(12):2485-2489. |
| APA | Ma Jun-Yan.,Chen Ke-Hai.,Zheng Xue-Fang.,Guo Ming.,Ma Jing.,...&Hu Jie-Han.(2007).Spectroscopy study on the interaction of colchicine and human serum albumin.spectroscopy and spectral analysis,27(12),2485-2489. |
| MLA | Ma Jun-Yan,et al."Spectroscopy study on the interaction of colchicine and human serum albumin".spectroscopy and spectral analysis 27.12(2007):2485-2489. |
入库方式： OAI收割
来源：大连化学物理研究所
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