Purification and in vitro cultivation of archaeocytes (stem cells) of the marine sponge Hymeniacidon perleve (Demospongiae)
文献类型：期刊论文
| 作者 | Sun, Liming; Song, Yuefan; Qu, Yi; Yu, Xingju; Zhang, Wei |
| 刊名 | cell and tissue research
 |
| 出版日期 | 2007-04-01 |
| 卷号 | 328期号:1页码:223-237 |
| 关键词 | archaeocyte invertebrate cell culture marine sponge Porifera Hymeniacidon perleve (Demospongiae) |
| 英文摘要 | marine sponges (porifera) are the best source of marine bioactive metabolites for drug discovery and development, although the sustainable production of most sponge-derived metabolites remains a difficult task. in vitro cultivation of sponge cells in bioreactors has been proposed as a promising technology. however, no continuous cell line has as yet been developed. archaeocytes are considered to be toti/multipotent stem cells in sponges and, when purified, may allow the development of continuous sponge cell lines. as a prerequisite, we have developed a novel four-step protocol for the purification of archaeocytes from a marine sponge, hymeniacidon perleve: (1) differential centrifugation to separate large sponge cells including archaeocytes; (2) selective agglomeration in low-ca2+/mg2+ artificial seawater in which living archaeocytes form small loose aggregates with some pinacocytes and collencytes; (3) differential adherence to remove anchorage-dependent pinacocytes, collencytes and other mesohyl cells; (4) ficoll-vrografin density gradient centrifugation to purify archaeocytes. the final purity of archaeocytes is greater than 80%. the proliferation potential of the archaeocytes has been demonstrated by high levels of brdu incorporation, pcna expression and telomerase activity. in 4-day primary cultures, the purified archaeocytes show a 2.5-fold increase in total cell number. this study opens an important avenue towards developing sponge cell cultures for the commercial exploitation of sponge- derived drugs. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | cell biology |
| 研究领域[WOS] | cell biology |
| 关键词[WOS] | dna polymerase-delta ; nuclear-protein cyclin ; auxiliary protein ; suberites-domuncula ; telomerase activity ; natural-products ; cellular-origin ; antigen ; cultures ; primmorphs |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000244888100020 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140524]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Bedford Pk, SA 5042, Australia |
| 推荐引用方式 GB/T 7714 | Sun, Liming,Song, Yuefan,Qu, Yi,et al. Purification and in vitro cultivation of archaeocytes (stem cells) of the marine sponge Hymeniacidon perleve (Demospongiae)[J]. cell and tissue research,2007,328(1):223-237. |
| APA | Sun, Liming,Song, Yuefan,Qu, Yi,Yu, Xingju,&Zhang, Wei.(2007).Purification and in vitro cultivation of archaeocytes (stem cells) of the marine sponge Hymeniacidon perleve (Demospongiae).cell and tissue research,328(1),223-237. |
| MLA | Sun, Liming,et al."Purification and in vitro cultivation of archaeocytes (stem cells) of the marine sponge Hymeniacidon perleve (Demospongiae)".cell and tissue research 328.1(2007):223-237. |
入库方式： OAI收割
来源：大连化学物理研究所
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