Preparation of polyacrylamide based monolith with immobilized pH gradient and its application for protein analysis
文献类型：期刊论文
| 作者 | Zhu GuiJie; Zhang WeiBing; Zhang LiHua; Liang Zhen; Zhang YuKui |
| 刊名 | science in china series b-chemistry
 |
| 出版日期 | 2007-08-01 |
| 卷号 | 50期号:4页码:526-529 |
| 关键词 | capillary isoelectric focusing monolith immobilized pH gradient proteins |
| 英文摘要 | monolithic materials were prepared in capillaries by in situ polymerization of acrylamide, glycidyl methacrylate and n,n'-memylenebisacrylamid in the presence of trinary porogens, including 1,4-butanediol, dodecanol and dimethyl sulphoxide. with ampholine immobilized on the monolith by chemical bonding according to their pis, the monolithic immobilized ph gradient (m-ipg) was prepared, and applied to the separation of four standard proteins. compared with polyacrylate based m-ipg, the hydrophilicity of the new material was improved. it could not only avoid the adsorption of proteins, but also make the synthesized procedure simple, which showed great potential in the analysis of proteins. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | carrier ampholytes |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000248663600012 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140552]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph R & A Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu GuiJie,Zhang WeiBing,Zhang LiHua,et al. Preparation of polyacrylamide based monolith with immobilized pH gradient and its application for protein analysis[J]. science in china series b-chemistry,2007,50(4):526-529. |
| APA | Zhu GuiJie,Zhang WeiBing,Zhang LiHua,Liang Zhen,&Zhang YuKui.(2007).Preparation of polyacrylamide based monolith with immobilized pH gradient and its application for protein analysis.science in china series b-chemistry,50(4),526-529. |
| MLA | Zhu GuiJie,et al."Preparation of polyacrylamide based monolith with immobilized pH gradient and its application for protein analysis".science in china series b-chemistry 50.4(2007):526-529. |
入库方式： OAI收割
来源：大连化学物理研究所
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