Screening and analysis of bioactive compounds in traditional Chinese medicines using cell extract and gas chromatography-mass spectrometry
文献类型：期刊论文
| 作者 | Zhang, Hx; Hu, C. X.; Liu, C. P.; Li, H. F.; Wang, J. S.; Yuan, K. L.; Tang, J. W.; Xu, G. W. |
| 刊名 | journal of pharmaceutical and biomedical analysis
 |
| 出版日期 | 2007-01-04 |
| 卷号 | 43期号:1页码:151-157 |
| 关键词 | bioactive compounds traditional Chinese medicines cell extract gas chromatography-mass spectrometer murine ascites hepatocarcinoma cell elemene emulsion injection zedoary turmeric oil and glucose injection |
| 英文摘要 | as the cost of drug development is always many times more than that of drug discovery, predictive methods aiding in the screening of bioavailable drug candidates are of profound significance. in this paper, a novel method for screening bioactive compounds from traditional chinese medicines (tcms) was developed by using living cell extract and gas chromatography (gc)-mass spectrometer (ms). the method was validated by using elemene emulsion injection (eei), a typical tcm with known active compound, to interact with murine ascites hepatocarcinoma cell strain with high metastatic potential (hca-f). finally, the method was applied to screen the bioactive compounds from multi-component zedoary turmeric oil and glucose injection (ztogi). after hca-f cells was incubated in ztogi, ethyl acetate (etoac) was used to extract the compounds in the cells for gc-ms analysis. fourteen compounds were detected in the desorption eluate of hca-f cell extract of ztogi, and further identified by ms. curzerene and beta-elemene were found to be two major bioactive compounds in ztogi. these results show that the method developed may be applied to quickly screen the potential bioactive components in tcms interacting with the target cells. (c) 2006 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences ; life sciences & biomedicine |
| 类目[WOS] | chemistry, analytical ; pharmacology & pharmacy |
| 研究领域[WOS] | chemistry ; pharmacology & pharmacy |
| 关键词[WOS] | immobilized liposome chromatography ; partition-coefficients ; membrane interactions ; angelica-sinensis ; zedoariae-rhizoma ; essential oil ; sesquiterpenes ; protein ; drugs ; phase |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000243554900018 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140564]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Dalian Med Univ, Dept Pathol, Dalian 116027, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Hx,Hu, C. X.,Liu, C. P.,et al. Screening and analysis of bioactive compounds in traditional Chinese medicines using cell extract and gas chromatography-mass spectrometry[J]. journal of pharmaceutical and biomedical analysis,2007,43(1):151-157. |
| APA | Zhang, Hx.,Hu, C. X..,Liu, C. P..,Li, H. F..,Wang, J. S..,...&Xu, G. W..(2007).Screening and analysis of bioactive compounds in traditional Chinese medicines using cell extract and gas chromatography-mass spectrometry.journal of pharmaceutical and biomedical analysis,43(1),151-157. |
| MLA | Zhang, Hx,et al."Screening and analysis of bioactive compounds in traditional Chinese medicines using cell extract and gas chromatography-mass spectrometry".journal of pharmaceutical and biomedical analysis 43.1(2007):151-157. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。