Axial temperature gradient and mobile phase gradient in microcolumn high-performance liquid chromatography
文献类型：期刊论文
| 作者 | Tian, Hongzhe; Xu, Jing; Guan, Yafeng |
| 刊名 | talanta
 |
| 出版日期 | 2007-04-30 |
| 卷号 | 72期号:2页码:813-818 |
| 关键词 | axial temperature gradient high temperature liquid chromatography micro-HPLC polycyclic aromatic hydrocarbons |
| 英文摘要 | the effect of axial temperature gradient (atg) along a microcolumn on the separation performance at both isocratic and gradient elution mode was investigated. a thermostat system was designed to form an atg along the packed column. polycyclic aromatic hydrocarbons (pahs) were separated on a 0.53 mm x 150 mm i.d. 5 mu m c-18 microcolumn, with water and acetonitrile as mobile phase. the separation results obtained at mobile phase gradient (mpg) and atg in microcolumn hplc were compared with the results performed at ambient conditions. extrapolated curves of peak width at half height (w(h)) versus ink showed that w(h) is narrower at the same retention time when atg was applied in addition to mpg. the column efficiency was enhanced 20-30% and the resolution was slightly reduced because of reduction of selectivity at elevated temperature at atg condition. the rsd of retention time in atg mode was less than 2.5%. (c) 2006 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | packed capillary columns ; hplc ; optimization ; separation ; retention |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000246321200070 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140589]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Dept Instrumentat & Analyt Sci, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tian, Hongzhe,Xu, Jing,Guan, Yafeng. Axial temperature gradient and mobile phase gradient in microcolumn high-performance liquid chromatography[J]. talanta,2007,72(2):813-818. |
| APA | Tian, Hongzhe,Xu, Jing,&Guan, Yafeng.(2007).Axial temperature gradient and mobile phase gradient in microcolumn high-performance liquid chromatography.talanta,72(2),813-818. |
| MLA | Tian, Hongzhe,et al."Axial temperature gradient and mobile phase gradient in microcolumn high-performance liquid chromatography".talanta 72.2(2007):813-818. |
入库方式： OAI收割
来源：大连化学物理研究所
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