Purification and characterization of a novel ginsenoside-hydrolyzing beta-D-glucosidase from the China white jade snail (Achatina fulica)
文献类型：期刊论文
| 作者 | Hu, Ying; Luan, Hongwei; Hao, Dacheng; Xiao, Hongbin; Yang, Shengli; Yang, Ling |
| 刊名 | enzyme and microbial technology
 |
| 出版日期 | 2007-04-03 |
| 卷号 | 40期号:5页码:1358-1366 |
| 关键词 | beta-D-glucosidase enzyme purification ginsenosides compound K China white jade snail |
| 英文摘要 | a beta-d-glucosidase, with broad regiospecific activity and designated as g h, was purified to homogeneity from the acetone power of viscera of the china white jade snail (achatina fulica). g ii consisted of two identical subunits (110 kda) with a native molecular mass of 220 kda. g ii was stable over a wide ph range (4-10 at 30 degrees c for 24 h) and at a relatively high temperature (50 degrees c for 8 h). moreover, it can cleave both beta-(1 -> 2)-glucosidic linkage at 3-c and beta-(1 -> 6)-glucosidic linkage at 20-c of ginsenosides and can hydrolyze ginsenosides rb-1, rb-2, rb-3 and rc into their active metabolites, compound k, compound y, mx, and mc, respectively. the optimal activity against p-nitrophenyl-beta-d-glucopyranoside (pnpg) was at 50 degrees c and ph 5.0. under the same condition, the k-m for pnpg was 0.224 mm with a y-max of 0.203 mmol nitrophenol min(-1) mg(-1). of the substrates tested, g ii specifically hydrolyzed the beta-d-glucosides involving aryl-beta-glucosides, alkyl-beta-glucosides, and beta-linked disaccharides (i.e. sophorose, gentiobiose, and cellobiose). these findings make the novel enzyme potentially useful in biotransformation processes as well as in the modification of multiple ginsenosides. (c) 2006 elsevier inc. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | anaerobic bacterium ; thermophilic fungus ; saponin metabolite ; compound-k ; in-vitro ; stability ; proteins ; cytotoxicity ; specificity ; enhancement |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000245519000053 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140590]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Lab Pharmaceut Resource Discovery, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Grad Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Med Chem Lab, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Ying,Luan, Hongwei,Hao, Dacheng,et al. Purification and characterization of a novel ginsenoside-hydrolyzing beta-D-glucosidase from the China white jade snail (Achatina fulica)[J]. enzyme and microbial technology,2007,40(5):1358-1366. |
| APA | Hu, Ying,Luan, Hongwei,Hao, Dacheng,Xiao, Hongbin,Yang, Shengli,&Yang, Ling.(2007).Purification and characterization of a novel ginsenoside-hydrolyzing beta-D-glucosidase from the China white jade snail (Achatina fulica).enzyme and microbial technology,40(5),1358-1366. |
| MLA | Hu, Ying,et al."Purification and characterization of a novel ginsenoside-hydrolyzing beta-D-glucosidase from the China white jade snail (Achatina fulica)".enzyme and microbial technology 40.5(2007):1358-1366. |
入库方式： OAI收割
来源：大连化学物理研究所
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