Over-expression, purification, and characterization of recombinant NAD-malic enzyme from Escherichia coli K12
文献类型：期刊论文
| 作者 | Wang, Jinxia; Tan, Haidong; Zhao, Zongbao (Kent) |
| 刊名 | protein expression and purification
 |
| 出版日期 | 2007-05-01 |
| 卷号 | 53期号:1页码:97-103 |
| 关键词 | malic enzyme Escherichia coli K12 over-expression enzyme kinetics |
| 英文摘要 | nad+-dependent malic enzyme (nad-me) gene from escherichia coli k12 was inserted into an expression vector pet24b(+) and transformed into e coli bl21 (de3). recombinant nad-me was expressed upon iptg induction, purified with affinity chromatography, and biochemically characterized. the results showed that recombinant nad-me could be produced mainly in a soluble form. the monomeric molecular weight of recombinant nad-me was about 65 kda, whereas monomer, homotetramer, and homooctamer were formed in solution as revealed by nondenaturing polyacrylamide gel electrophoresis analysis. finally, the k-m, values of nad-me for l-malate and nad were determined as 0.420 +/- 0.174 and 0.097 +/- 0.038 mm, respectively, at ph 7.2. by using this over-expression and purification system, recombinant e coli k12 nad-me can now be obtained in large quantity necessary for further biochemical characterization and applications. (c) 2006 elsevier inc. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemical research methods ; biochemistry & molecular biology ; biotechnology & applied microbiology |
| 研究领域[WOS] | biochemistry & molecular biology ; biotechnology & applied microbiology |
| 关键词[WOS] | polyacrylamide-gel electrophoresis ; molecular-weights ; coli ; binding ; cloning ; gene ; site ; residues ; pigeon |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000246245000011 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140630]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Jinxia,Tan, Haidong,Zhao, Zongbao . Over-expression, purification, and characterization of recombinant NAD-malic enzyme from Escherichia coli K12[J]. protein expression and purification,2007,53(1):97-103. |
| APA | Wang, Jinxia,Tan, Haidong,&Zhao, Zongbao .(2007).Over-expression, purification, and characterization of recombinant NAD-malic enzyme from Escherichia coli K12.protein expression and purification,53(1),97-103. |
| MLA | Wang, Jinxia,et al."Over-expression, purification, and characterization of recombinant NAD-malic enzyme from Escherichia coli K12".protein expression and purification 53.1(2007):97-103. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。