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High-density cultivation of oleaginous yeast Rhodosporidium toruloides Y4 in fed-batch culture
文献类型：期刊论文
| 作者 | Li, Yonghong; Zhao, Zongbao (Kent); Bai, Fengwu |
| 刊名 | enzyme and microbial technology
 |
| 出版日期 | 2007-08-02 |
| 卷号 | 41期号:3页码:312-317 |
| 关键词 | high-density cell culture Rhorosporidium toruloides Y4 fed-batch culture microbial lipid |
| 英文摘要 | microbial lipid production by the oleaginous yeast rhorosporidium toruloides y4 was studied using glucose as carbon source, in order to realize high-density cell culture. batch cultures demonstrated that there was little inhibitory effect with a substrate concentration of up to 150 g l(-1). flask fed-batch cultures were run for 25 days and reached a dry biomass and cellular lipid content of 151.5 g l(-1) and 48.0% (w/w), respectively. using pilot-scale fed-batch cultures in a 15-1 stirred-tank fermenter for 134 h resulted in dry biomass, lipid content and lipid productivity of 106.5 g l(-1), 67.5% (w/w) and 0.54 g l(-1) h(-1), respectively. the fed-batch culture model used here featured initial nutrient-rich media and a pure carbon source with discontinuous feeding. gas chromatography analysis revealed that lipids from r. toruloides y4 contained mainly long-chain fatty acids with 16 and 18 carbon atoms. the four major constituent fatty acids were oleic acid, palmitic acid, stearic acid and linoleic acid. a slight increase in stearic acid production was found during the culture process. based on these compositional data, microbial lipids from r. toruloides y4 are a potential alternative oil resource for biodiesel production. (c) 2007 elsevier inc. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | yarrowia-lipolytica ; lipid-accumulation ; apiotrichum-curvatum ; carbon ; acid ; glucose |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000247360900018 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140689]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Univ Technol, Dept Biosci & Bioengn, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yonghong,Zhao, Zongbao ,Bai, Fengwu. High-density cultivation of oleaginous yeast Rhodosporidium toruloides Y4 in fed-batch culture[J]. enzyme and microbial technology,2007,41(3):312-317. |
| APA | Li, Yonghong,Zhao, Zongbao ,&Bai, Fengwu.(2007).High-density cultivation of oleaginous yeast Rhodosporidium toruloides Y4 in fed-batch culture.enzyme and microbial technology,41(3),312-317. |
| MLA | Li, Yonghong,et al."High-density cultivation of oleaginous yeast Rhodosporidium toruloides Y4 in fed-batch culture".enzyme and microbial technology 41.3(2007):312-317. |
入库方式： OAI收割
来源：大连化学物理研究所
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