Monolith-based immobilized enzyme reactors: Recent developments and applications for proteome analysis
文献类型：期刊论文
| 作者 | Ma, Junfeng; Zhang, Lihua; Liang, Zhen; Zhang, Weibing; Zhang, Yukui |
| 刊名 | journal of separation science
 |
| 出版日期 | 2007-11-01 |
| 卷号 | 30期号:17页码:3050-3059 |
| 关键词 | immobilized enzyme reactor monolith proteome |
| 英文摘要 | in the current proteome study, protein digestion is indispensable before proteins could be identified by ms/ms, no matter based on top-down or bottom-up strategies. compared to the traditional digestion performed in free solution, immobilized enzyme shows great advantages in digestion speed, stability, and longevity, especially with monolithic materials as the supports. besides the improved digestion capacity, the immobilized enzyme reactors (imers) could be further coupled with the separation and detection systems, enabling high-throughput protein identification. in this paper, the latest advances in the monolith-based imers and their applications in proteomic study are briefly summarized. by reviewing these achievements, it could be seen that monilith-based imers have very bright future in proteome analysis. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | performance liquid-chromatography ; tandem mass-spectrometry ; peptide-mapping analysis ; less common applications ; solid-phase extraction ; capillary-electrophoresis ; trypsin microreactor ; affinity-chromatography ; proteolytic digestion ; microfluidic devices |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000251461500033 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140724]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatogrph R&A Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Junfeng,Zhang, Lihua,Liang, Zhen,et al. Monolith-based immobilized enzyme reactors: Recent developments and applications for proteome analysis[J]. journal of separation science,2007,30(17):3050-3059. |
| APA | Ma, Junfeng,Zhang, Lihua,Liang, Zhen,Zhang, Weibing,&Zhang, Yukui.(2007).Monolith-based immobilized enzyme reactors: Recent developments and applications for proteome analysis.journal of separation science,30(17),3050-3059. |
| MLA | Ma, Junfeng,et al."Monolith-based immobilized enzyme reactors: Recent developments and applications for proteome analysis".journal of separation science 30.17(2007):3050-3059. |
入库方式： OAI收割
来源：大连化学物理研究所
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