Enhancing the activity and regioselectivity of lipases for 3 '-benzoylation of floxuridine and its analogs by using ionic liquid-containing systems
文献类型：期刊论文
| 作者 | Li, Ning1,2; Ma, Ding; Zong, Min-Hua2 |
| 刊名 | journal of biotechnology
 |
| 出版日期 | 2008 |
| 卷号 | 133期号:1页码:103-109 |
| 关键词 | enzymatic benzoylation lipase ionic liquid-containing systems regioselectivity floxuridine and its analogs vinyl benzoate |
| 英文摘要 | ionic liquid-containing systems offered new opportunities to enzymatic acylation of nucleosides. in pseudomonas cepacia lipase-mediated benzoylation of floxuricline (fudr), the enzyme performances, including enzyme activity and 3'-regioselectivity, were significantly enhanced by using [c(4)mim]pf6-containing systems. it was observed that the enzyme performances not only depended on the anion of il, but also on the cation, and that a proper combination between the cation and anion was critical to allow the enzyme to exhibit excellent performances. the optimal il content in il-containing systems is 5% (v/v). to gain a deeper insight into enzyme recognition, we extended benzoylation of fudr catalyzed by p cepacia lipase to its analogs. fudr and its analogs could be biotransformed to the desired product by r cepacia lipase in excellent conversion (>99%) and good to excellent 3'-regioselectivity (81-99%). furthermore, the 3'-regioselectivity of p. cepacia lipase was enhanced with the increase of hydrophobicity of 5-substituent of the substrates, due to better hydrophobic interactions with leu287 present in the active site. (c) 2007 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | acylation ; efficient ; benzoylation ; biocatalysis ; nucleosides ; solvent |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000251853100014 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140751]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China 2.S China Univ Technol, Lab Appl Biocatalysis, Guangzhou 510640, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Ning,Ma, Ding,Zong, Min-Hua. Enhancing the activity and regioselectivity of lipases for 3 '-benzoylation of floxuridine and its analogs by using ionic liquid-containing systems[J]. journal of biotechnology,2008,133(1):103-109. |
| APA | Li, Ning,Ma, Ding,&Zong, Min-Hua.(2008).Enhancing the activity and regioselectivity of lipases for 3 '-benzoylation of floxuridine and its analogs by using ionic liquid-containing systems.journal of biotechnology,133(1),103-109. |
| MLA | Li, Ning,et al."Enhancing the activity and regioselectivity of lipases for 3 '-benzoylation of floxuridine and its analogs by using ionic liquid-containing systems".journal of biotechnology 133.1(2008):103-109. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。