Design a chemically defined/medically approved medium for cell transplantation according to the metabolic characteristics of microencapsulated cells and the process of encapsulation
文献类型：期刊论文
| 作者 | Lv, Guojun1,2; Sun, Zhijie1,2; Li, Na3; Li, Shuangyue1,2; Zhang, Ying1,2; Xie, Yubing1; Yu, Weiting1,2; Wang, Wei1; Ma, Xiaojun1 |
| 刊名 | biochemical engineering journal
 |
| 出版日期 | 2008-04-01 |
| 卷号 | 39期号:1页码:157-163 |
| 关键词 | cell transplantation microencapsulation recombinant CHO cell chemically defined medium amino acid |
| 英文摘要 | cell microencapsulation is a promising therapeutic strategy to many diseases; however, the metabolic characteristics of microencapsulated cells and also the optimum microcapsule additives that meet the criteria for long-term functional immunoisolated transplantation have rarely been taken into consideration hitherto. in this work, the optimized amino acid supplements and several chemically defined compounds were applied to cultivation of the microencapsulated cho cells. the results show that the optimized medium significantly enhances cell survival and, particularly, endostatin secretion of encapsulated cho cells over a time period of 8 weeks. furthermore, long-term in vitro stability of alginate-poly-llysine-alginate (apa) microcapsules underwent no changes, which indicated that this optimized medium might be feasible to maintain high viability of microencapsulated cell for short periods of time before implantation. (c) 2007 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; technology |
| 类目[WOS] | biotechnology & applied microbiology ; engineering, chemical |
| 研究领域[WOS] | biotechnology & applied microbiology ; engineering |
| 关键词[WOS] | monoclonal-antibody production ; human serum-albumin ; protein-free medium ; lysophosphatidic acid ; phosphatidic-acid ; hybridoma growth ; apa microcapsule ; culture ; alginate ; proliferation |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000254479700019 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140827]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Jilin Agr Univ, Grad Sch, Changchun 130118, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lv, Guojun,Sun, Zhijie,Li, Na,et al. Design a chemically defined/medically approved medium for cell transplantation according to the metabolic characteristics of microencapsulated cells and the process of encapsulation[J]. biochemical engineering journal,2008,39(1):157-163. |
| APA | Lv, Guojun.,Sun, Zhijie.,Li, Na.,Li, Shuangyue.,Zhang, Ying.,...&Ma, Xiaojun.(2008).Design a chemically defined/medically approved medium for cell transplantation according to the metabolic characteristics of microencapsulated cells and the process of encapsulation.biochemical engineering journal,39(1),157-163. |
| MLA | Lv, Guojun,et al."Design a chemically defined/medically approved medium for cell transplantation according to the metabolic characteristics of microencapsulated cells and the process of encapsulation".biochemical engineering journal 39.1(2008):157-163. |
入库方式： OAI收割
来源：大连化学物理研究所
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