Glass microfluidic chip-based sodium dodecyl sulfate-nongel sieving electrophoresis for determining molecular weights of immunoglobulin fragments
文献类型：期刊论文
| 作者 | Huang Huai-Qing; Dai Zhong-Peng; Qin Jian-Hua; Lin Bing-Cheng |
| 刊名 | chinese journal of analytical chemistry
 |
| 出版日期 | 2008-06-01 |
| 卷号 | 36期号:6页码:719-723 |
| 关键词 | microfluidic chip sodium dodecyl sulfate-nongel sieving electrophoresis protein immunoglobulin molecular weight |
| 英文摘要 | the concentrations of dextran sieving matrix and running buffer were optimized. under the optimized conditions (10% (w/v) dextran, 0.1% sds, 10% glycerol, 0.2 mol/l tris-boric acid, ph 8.3), six standard proteins could be efficiently separated using sodium dodecyl sulfate (sds)-nongel sieving electrophoresis on home-made glass microfluidic chip. the relative standard deviations (rsds) for migration time were lower than 0.50%. a good linear relationship (r =0.994) was built between the migration time of each protein and the logarithm of its molecular weight (logmr). using the chip-based electrophoretic separation system, the molecular weights of different fragments of immunoglobulin g after different treatments were measured, and the results matched the facts. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | capillary gel-electrophoresis ; monoclonal-antibody ; microchip |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000257877300001 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140837]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem & Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang Huai-Qing,Dai Zhong-Peng,Qin Jian-Hua,et al. Glass microfluidic chip-based sodium dodecyl sulfate-nongel sieving electrophoresis for determining molecular weights of immunoglobulin fragments[J]. chinese journal of analytical chemistry,2008,36(6):719-723. |
| APA | Huang Huai-Qing,Dai Zhong-Peng,Qin Jian-Hua,&Lin Bing-Cheng.(2008).Glass microfluidic chip-based sodium dodecyl sulfate-nongel sieving electrophoresis for determining molecular weights of immunoglobulin fragments.chinese journal of analytical chemistry,36(6),719-723. |
| MLA | Huang Huai-Qing,et al."Glass microfluidic chip-based sodium dodecyl sulfate-nongel sieving electrophoresis for determining molecular weights of immunoglobulin fragments".chinese journal of analytical chemistry 36.6(2008):719-723. |
入库方式： OAI收割
来源：大连化学物理研究所
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