Characterization of multi-phosphopeptides by mu HPLC-ESI-MS/MS with a alkaline phosphatase treatment
文献类型：期刊论文
| 作者 | Wang, Hui1,2; Duan, Jicheng1,2; Zhang, Lihua1; Liang, Zhen1; Zhang, Weibing1; Zhang, Yukui1 |
| 刊名 | journal of separation science
 |
| 出版日期 | 2008-02-01 |
| 卷号 | 31期号:3页码:480-487 |
| 关键词 | alkaline phosphatase dephosphorylation multi-phosphopeptide IHPLC-ESI-MS/MS |
| 英文摘要 | the detection of phosphopeptides, especially multi-phosphopeptides, by tandem electrospray ionization mass spectrometry (esi-ms/ms) is a great challenge due to their low abundance and the poor ionization efficiency of samples. in our recent study, a strategy was proposed for the analysis of trace multi-phosphopeptides which combined selective enrichment of phosphorylated peptides by tio2 and dephosphorylation by alkaline phosphatase (ap). after separation by mu hplc, the profiles of enriched peptides before and after ap treatment were compared, and the additional peaks appearing in the latter case hinted at the existence of multi-phosphopeptides. subsequently, an incomplete dephosphorylation reaction was performed to partially remove the phosphate groups so that the phosphorylation sites of the multi-phosphopeptides might be estimated. through analysis of the digests of beta-casein and extracted proteins of bovine milk, more information on the multi-phosphopeptides was obtained by mu hplc-esi-ms/ms than that obtained without ap treatment, which demonstrated that such a strategy might supply some potential information about trace multi-phosphopeptides lost in shotgun analysis. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | ion affinity-chromatography ; mass-spectrometry ; protein-phosphorylation ; maldi ms ; enrichment ; identification ; peptides ; phosphoproteins ; nanoparticles ; proteomics |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000253689700006 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140870]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Hui,Duan, Jicheng,Zhang, Lihua,et al. Characterization of multi-phosphopeptides by mu HPLC-ESI-MS/MS with a alkaline phosphatase treatment[J]. journal of separation science,2008,31(3):480-487. |
| APA | Wang, Hui,Duan, Jicheng,Zhang, Lihua,Liang, Zhen,Zhang, Weibing,&Zhang, Yukui.(2008).Characterization of multi-phosphopeptides by mu HPLC-ESI-MS/MS with a alkaline phosphatase treatment.journal of separation science,31(3),480-487. |
| MLA | Wang, Hui,et al."Characterization of multi-phosphopeptides by mu HPLC-ESI-MS/MS with a alkaline phosphatase treatment".journal of separation science 31.3(2008):480-487. |
入库方式： OAI收割
来源：大连化学物理研究所
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