Direct electron transfer of horseradish peroxidase and its biosensor based on gold nanoparticles/chitosan/ITO modified electrode
文献类型：期刊论文
| 作者 | Xiang, Cuili2; Zou, Yongjin2; Sun, Li-Xian1; Xu, Fen |
| 刊名 | analytical letters
 |
| 出版日期 | 2008 |
| 卷号 | 41期号:12页码:2224-2236 |
| 关键词 | chitosan direct electron transfer gold nanoparticles horseradish peroxidase ITO electrode |
| 英文摘要 | gold nanoparticles/chitosan modified indium tin oxide (ito) coated glass electrode was prepared by a simple layer-by-layer self-assembly technique. horseradish peroxidase (hrp) was successfully immobilized on the modified electrode by electrostatic adsorption. direct electrochemistry and electrocatalysis of hrp were investigated. the composite film showed an obvious promotion for the direct electron transfer between hrp and the underlying electrode. the immobilized hrp exhibited an excellent electrocatalytic activity toward the reduction of h(2)o(2). the catalysis currents increased linearly to the h(2)o(2) concentration in a wide range of 2.0 x 10(-5) to 6.5 x 10(-3) m with a low detection limit of 3.5 x 10(-6) m. the calculated apparent michaelis-menten constant (k(m)(app)) was 4.43mm. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | direct electrochemistry ; hydrogen-peroxide ; cytochrome-c ; electrocatalysis ; nanocomposite ; immobilization ; voltammetry ; proteins ; chitosan ; enzymes |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000258487500009 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140874]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Mat & Thermochem Lab, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiang, Cuili,Zou, Yongjin,Sun, Li-Xian,et al. Direct electron transfer of horseradish peroxidase and its biosensor based on gold nanoparticles/chitosan/ITO modified electrode[J]. analytical letters,2008,41(12):2224-2236. |
| APA | Xiang, Cuili,Zou, Yongjin,Sun, Li-Xian,&Xu, Fen.(2008).Direct electron transfer of horseradish peroxidase and its biosensor based on gold nanoparticles/chitosan/ITO modified electrode.analytical letters,41(12),2224-2236. |
| MLA | Xiang, Cuili,et al."Direct electron transfer of horseradish peroxidase and its biosensor based on gold nanoparticles/chitosan/ITO modified electrode".analytical letters 41.12(2008):2224-2236. |
入库方式： OAI收割
来源：大连化学物理研究所
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