Matrix molecularly imprinted polymer for enriching lysozyme in protein samples
文献类型:期刊论文
作者 | Liu Qiu-Ye1; Gai Qing-Qing1; He Xi-Wen1; Li Wen-You1; Chen Lang-Xing1; Zhang Yu-Kui1,2 |
刊名 | chemical journal of chinese universities-chinese
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出版日期 | 2008-03-10 |
卷号 | 29期号:3页码:505-509 |
关键词 | molecular imprinting polymer lysozyme silica polyacrylamide gel |
英文摘要 | the effects of the molar ratio of functional monomer to template protein, the ph and the ionic strength of the medium on the interaction between the functional monomers and template protein were primarily investigated from the molecular level by uv spectrophotometry. then lysozyme(lyz) -imprinted polyacrylamide gel was prepared to recognize effectively lyz in protein samples. with acrylamide and n,n-methylenediacryl-amide as the polymeric matrix and sillca as the pore-forming reagent, after template and silica were removed, the surface of gel polymer had a large quantity of well-distributed macropores, which were suited to let the proteins pass in and out. the recognition and selectivity of the imprinted polymers were studied with equilibrium adsorption. selective adsorption experiments of the imprinted gel show that imprinted gel exhibited good recognition capability for lyz compared with bovine serum albumin or ovalbum as the completive substance. |
WOS标题词 | science & technology ; physical sciences |
类目[WOS] | chemistry, multidisciplinary |
研究领域[WOS] | chemistry |
关键词[WOS] | solid-phase extraction ; chinese traditional medicine ; polyacrylamide-gel beads ; bovine serum-albumin ; recognition ; templates ; chromatography ; antibodies ; nanowires |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000254265100015 |
公开日期 | 2015-11-17 |
源URL | [http://159.226.238.44/handle/321008/140894] ![]() |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
作者单位 | 1.Nankai Univ, Coll Chem, Tianjin 300071, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China |
推荐引用方式 GB/T 7714 | Liu Qiu-Ye,Gai Qing-Qing,He Xi-Wen,et al. Matrix molecularly imprinted polymer for enriching lysozyme in protein samples[J]. chemical journal of chinese universities-chinese,2008,29(3):505-509. |
APA | Liu Qiu-Ye,Gai Qing-Qing,He Xi-Wen,Li Wen-You,Chen Lang-Xing,&Zhang Yu-Kui.(2008).Matrix molecularly imprinted polymer for enriching lysozyme in protein samples.chemical journal of chinese universities-chinese,29(3),505-509. |
MLA | Liu Qiu-Ye,et al."Matrix molecularly imprinted polymer for enriching lysozyme in protein samples".chemical journal of chinese universities-chinese 29.3(2008):505-509. |
入库方式: OAI收割
来源:大连化学物理研究所
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