Phenotype differentiation of three E-coli strains by GC-FID and GC-MS based metabolomics
文献类型：期刊论文
| 作者 | Tian, Jing2; Shi, Chunyun2; Gao, Peng; Yuan, Kailong; Yang, Dawei2; Lu, Xin; Xu, Guowang1 |
| 刊名 | journal of chromatography b-analytical technologies in the biomedical and life sciences
 |
| 出版日期 | 2008-08-15 |
| 卷号 | 871期号:2页码:220-226 |
| 关键词 | metabolomics E. coli phenotype GC-FID GC-MS |
| 英文摘要 | two mutants of e call with deletion of sdhab and acka-pta genes respectively and their wild-type strains were subjected to gas chromatography-flame-ionization detection (gc-fid) and gas chromatography-mass spectrometry (gc-ms) metabolomics analysis. intracellular metabolites of the three strains were profiled by gc-fid firstly. methodological evaluation of the employed platform indicated that the limit of detection ranges were from 0.2 to 12.5 ng for some representative metabolites and the corresponding recoveries were varied from 68.7 to 122.7%. secondly, multivariable data analysis was applied to the acquired data sets. as expected, the three phenotypes could be easily differentiated, and the perturbed metabolite pools in the genetically modified strains were screened. lastly, the metabolites playing key roles in the differentiation were further identified by gc-ms. it was confirmed that succinic acid and aspartic acid were similarly affected in the modified strains. but proline content was altered contrarily. additionally, deletion of sdhab gene also affected the growth property of relevant mutant greatly. the potential mechanism was postulated accordingly. (c) 2008 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | solid-phase microextraction ; systems biology ; functional genomics ; headspace-gc ; spectrometry ; networks ; strategy ; enzymes ; growth ; water |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000259130400009 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140962]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Dalian Polytech Univ, Dept Modern Technol, Dalian 116034, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tian, Jing,Shi, Chunyun,Gao, Peng,et al. Phenotype differentiation of three E-coli strains by GC-FID and GC-MS based metabolomics[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2008,871(2):220-226. |
| APA | Tian, Jing.,Shi, Chunyun.,Gao, Peng.,Yuan, Kailong.,Yang, Dawei.,...&Xu, Guowang.(2008).Phenotype differentiation of three E-coli strains by GC-FID and GC-MS based metabolomics.journal of chromatography b-analytical technologies in the biomedical and life sciences,871(2),220-226. |
| MLA | Tian, Jing,et al."Phenotype differentiation of three E-coli strains by GC-FID and GC-MS based metabolomics".journal of chromatography b-analytical technologies in the biomedical and life sciences 871.2(2008):220-226. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。