A glycerol assisted light-emitting diode-induced fluorescence detector for capillary flow systems
文献类型：期刊论文
| 作者 | Xu, Jing; Chen, Shiheng; Xiong, Yan; Yang, Bingcheng; Guan, Yafeng |
| 刊名 | talanta
 |
| 出版日期 | 2008-05-30 |
| 卷号 | 75期号:4页码:885-889 |
| 关键词 | light-emitting diode fluorescence detection capillary electrophoresis fefractive index matching fluid |
| 英文摘要 | a glycerol assisted light-emitting diode (led)-induced fluorescence detector (if) for capillary flow systems was constructed and evaluated. a blue led was used as the excitation source, and optical fibers (of) were used to transmit the excitation light and collect the fluorescence. a commercial available 5-port manifold was used as detection cell, where the capillary tube and the of were fixed into the manifold. the precision of the holes on the manifold ensured a self-alignment of optical path. a refractive index matching fluid (rimf)-glycerol was used to eliminate the interfaces between the of and the led, as well as between the fused silica capillary and the transmitting/collecting fiber. the enhancement of excitation light led to 2.8-folds improvement on the signal-to-noise ratio. the use of rimf also eliminates focusing effect of the capillary wall and reduces both the excitation light directed to the detection cell and background signal, resulting in reduction in the fluorescence intensity and noise level. the intensity was reduced to 47-63% for laser and 60-77% for led, respectively, for capillaries with i.d. from 50 to 250 mu m; while the noise level was reduced to 1/3 when rimf was used for both laser and led on the tested capillaries. about 5.6-fold enhancement in signal-to-noise ratio was obtained in total. the detection limit of the led-if for fluorescein isothiocyanate (fitc) was 4 nm. application of the led-if for the analysis of fitc-labeled amino acids by electrophoresis was demonstrated. (c) 2008 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | electrophoresis system ; array electrophoresis ; injection ; cell ; sample |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000256208200003 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/140976]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem & Microinstrumentat, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Jing,Chen, Shiheng,Xiong, Yan,et al. A glycerol assisted light-emitting diode-induced fluorescence detector for capillary flow systems[J]. talanta,2008,75(4):885-889. |
| APA | Xu, Jing,Chen, Shiheng,Xiong, Yan,Yang, Bingcheng,&Guan, Yafeng.(2008).A glycerol assisted light-emitting diode-induced fluorescence detector for capillary flow systems.talanta,75(4),885-889. |
| MLA | Xu, Jing,et al."A glycerol assisted light-emitting diode-induced fluorescence detector for capillary flow systems".talanta 75.4(2008):885-889. |
入库方式： OAI收割
来源：大连化学物理研究所
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