Biological fingerprinting analysis of interaction between taxoids in Taxus and microtubule protein by microdialysis coupled, with high-performance liquid chromatography/mass spectrometry for screening antimicrotubule agents
文献类型：期刊论文
| 作者 | Lei Xiao-yuan1; Kong Liang2; Su Xing-ye1; Guo Ming3; Zou Han-fa1 |
| 刊名 | chemical research in chinese universities
 |
| 出版日期 | 2008-07-01 |
| 卷号 | 24期号:4页码:411-419 |
| 关键词 | microdialysis/high-performance liquid chromatography(HPLC) biological fingerprinting analysis taxoids microtubule Taxus |
| 英文摘要 | some natural products, such as traditional chinese medicines(tcms), contain compounds with anticancer activity and have attracted a great interest in recent years as alternative anticancer therapies. a quick and convenient assay for screening antimicrotubule compounds in which in vitro microdialysis/high-performance liquid chromatography (hplc) is used to monitor the binding of the compounds extracted from tcm taxus cuspidata siebold & zucc(taxus) to microtubules is reported. it was observed that the extract of taxus contains at least five compounds which have affinity interaction with microtubules by biological fingerprinting analysis, and they were identified as the taxoids of taxol, baccatin iii, 10-deacetylbaccatin iii(10-dab), cephalomannine and 7-epi-10-deacetyltaxol (7-epi-10-dat) based on the comparison of their high-performance liquid chromatographic/mass spectrometric and uv spectra with those of the standard samples, both assembly-promoting and disassembly-inhibiting characteristics of those compounds were evaluated. it was observed that baccatin ill and 10-dab bound to microtubules and the binding degrees were influenced by gtp. competitive binding behavior of taxol with other four taxoids to microtubules was also investigated. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | traditional chinese medicines ; japanese yew ; bioactive compounds ; tubulin ; cuspidata ; drugs ; chemotherapy ; modulation ; invitro ; target |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000258446000006 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141005]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Dalian Fisheries Univ, Coll Marine Environm Engn, Dalian 116023, Peoples R China 3.Dalian Univ, Dept Chem & Chem Technol, Dalian 116622, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lei Xiao-yuan,Kong Liang,Su Xing-ye,et al. Biological fingerprinting analysis of interaction between taxoids in Taxus and microtubule protein by microdialysis coupled, with high-performance liquid chromatography/mass spectrometry for screening antimicrotubule agents[J]. chemical research in chinese universities,2008,24(4):411-419. |
| APA | Lei Xiao-yuan,Kong Liang,Su Xing-ye,Guo Ming,&Zou Han-fa.(2008).Biological fingerprinting analysis of interaction between taxoids in Taxus and microtubule protein by microdialysis coupled, with high-performance liquid chromatography/mass spectrometry for screening antimicrotubule agents.chemical research in chinese universities,24(4),411-419. |
| MLA | Lei Xiao-yuan,et al."Biological fingerprinting analysis of interaction between taxoids in Taxus and microtubule protein by microdialysis coupled, with high-performance liquid chromatography/mass spectrometry for screening antimicrotubule agents".chemical research in chinese universities 24.4(2008):411-419. |
入库方式： OAI收割
来源：大连化学物理研究所
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