中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Preparation of bovine hemoglobin-imprinted polymer beads via photografting surface-modified method

文献类型:期刊论文

作者Gai Qing-Qing1,3; Liu Qiu-Ye1; Li Wen-You1; He Xi-Wen1; Chen Lang-Xing1; Zhang Yu-Kui1,2
刊名chemical journal of chinese universities-chinese
出版日期2008-01-10
卷号29期号:1页码:64-70
关键词molecular imprinting molecular recognition bovine hemoglobin photografting iniferter
英文摘要molecularly imprinted polymers(mip) based on photografting surface-modified polystyrene beads as matrices were prepared with acrylamide as the functional monomer, bovine hemoglobin as the template molecule and n, n'-methylene bisacrylamide as the crosslinker in a phosphate buffer. the results of ir, scanning electron microscope(sem) and elemental analysis demonstrate the formation of a grafting polymer layer on the polystyrene-bead surface. subsequent removal of the template leaves behind cavities on the surface of polymer matrix with a shape and an arrangement of functional groups in complementary binding sites to the original template. and the adsorption studies show that the imprinted polymers have a good adsorption capacity and specific recognition for bovine hemoglobin as the template molecule. our results demonstrated that the polymer prepared via photografting surface-modified method exhibited a better selectivity for the template. attempt to employ the new method in molecular imprinting techniques may indroduce new application to mips and facillcate the probable protein separation and purification.
WOS标题词science & technology ; physical sciences
类目[WOS]chemistry, multidisciplinary
研究领域[WOS]chemistry
关键词[WOS]solid-phase extraction ; liquid-chromatography ; recognition ; protein ; albumin ; sensor ; acid
收录类别SCI
语种英语
WOS记录号WOS:000252618000013
公开日期2015-11-17
源URL[http://159.226.238.44/handle/321008/141032]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
作者单位1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
3.Changzhi Med Coll, Dept Pharmacol, Changzhi 046000, Peoples R China
推荐引用方式
GB/T 7714
Gai Qing-Qing,Liu Qiu-Ye,Li Wen-You,et al. Preparation of bovine hemoglobin-imprinted polymer beads via photografting surface-modified method[J]. chemical journal of chinese universities-chinese,2008,29(1):64-70.
APA Gai Qing-Qing,Liu Qiu-Ye,Li Wen-You,He Xi-Wen,Chen Lang-Xing,&Zhang Yu-Kui.(2008).Preparation of bovine hemoglobin-imprinted polymer beads via photografting surface-modified method.chemical journal of chinese universities-chinese,29(1),64-70.
MLA Gai Qing-Qing,et al."Preparation of bovine hemoglobin-imprinted polymer beads via photografting surface-modified method".chemical journal of chinese universities-chinese 29.1(2008):64-70.

入库方式: OAI收割

来源:大连化学物理研究所

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