Preparation of bovine hemoglobin-imprinted polymer beads via photografting surface-modified method
文献类型:期刊论文
作者 | Gai Qing-Qing1,3; Liu Qiu-Ye1; Li Wen-You1; He Xi-Wen1; Chen Lang-Xing1; Zhang Yu-Kui1,2 |
刊名 | chemical journal of chinese universities-chinese
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出版日期 | 2008-01-10 |
卷号 | 29期号:1页码:64-70 |
关键词 | molecular imprinting molecular recognition bovine hemoglobin photografting iniferter |
英文摘要 | molecularly imprinted polymers(mip) based on photografting surface-modified polystyrene beads as matrices were prepared with acrylamide as the functional monomer, bovine hemoglobin as the template molecule and n, n'-methylene bisacrylamide as the crosslinker in a phosphate buffer. the results of ir, scanning electron microscope(sem) and elemental analysis demonstrate the formation of a grafting polymer layer on the polystyrene-bead surface. subsequent removal of the template leaves behind cavities on the surface of polymer matrix with a shape and an arrangement of functional groups in complementary binding sites to the original template. and the adsorption studies show that the imprinted polymers have a good adsorption capacity and specific recognition for bovine hemoglobin as the template molecule. our results demonstrated that the polymer prepared via photografting surface-modified method exhibited a better selectivity for the template. attempt to employ the new method in molecular imprinting techniques may indroduce new application to mips and facillcate the probable protein separation and purification. |
WOS标题词 | science & technology ; physical sciences |
类目[WOS] | chemistry, multidisciplinary |
研究领域[WOS] | chemistry |
关键词[WOS] | solid-phase extraction ; liquid-chromatography ; recognition ; protein ; albumin ; sensor ; acid |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000252618000013 |
公开日期 | 2015-11-17 |
源URL | [http://159.226.238.44/handle/321008/141032] ![]() |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
作者单位 | 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China 3.Changzhi Med Coll, Dept Pharmacol, Changzhi 046000, Peoples R China |
推荐引用方式 GB/T 7714 | Gai Qing-Qing,Liu Qiu-Ye,Li Wen-You,et al. Preparation of bovine hemoglobin-imprinted polymer beads via photografting surface-modified method[J]. chemical journal of chinese universities-chinese,2008,29(1):64-70. |
APA | Gai Qing-Qing,Liu Qiu-Ye,Li Wen-You,He Xi-Wen,Chen Lang-Xing,&Zhang Yu-Kui.(2008).Preparation of bovine hemoglobin-imprinted polymer beads via photografting surface-modified method.chemical journal of chinese universities-chinese,29(1),64-70. |
MLA | Gai Qing-Qing,et al."Preparation of bovine hemoglobin-imprinted polymer beads via photografting surface-modified method".chemical journal of chinese universities-chinese 29.1(2008):64-70. |
入库方式: OAI收割
来源:大连化学物理研究所
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